The oral protective efficacy of magnolol against Aeromonas hydrophila and A. veronii infection via enhancing anti-inflammatory ability in goldfish (Carassius auratus).
The oral protective efficacy of magnolol against Aeromonas hydrophila and A. veronii infection via enhancing anti-inflammatory ability in goldfish (Carassius auratus).
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DOI：
10.1111/jfd.13859
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发表时间：
2023-09
影响因子：
2.5
通讯作者：
Zhao Zhang;Jing Li;Gaoxue Wang;F. Ling
中科院分区：
文献类型：
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作者：
Zhao Zhang;Jing Li;Gaoxue Wang;F. Ling
嗜水气单胞菌和维罗尼气单胞菌是水产养殖业中广泛存在的重要致病菌，会造成严重的经济损失。目前，厚朴酚已被证明具有广谱抗菌活性，如嗜水菌、金黄色葡萄球菌、变形链球菌等。为探讨厚朴酚体内抗病原因，促进其在水产养殖中的安全应用，本文对投喂厚朴酚的鱼类进行病理检测及免疫指标变化。结果表明，添加厚朴酚的饲料（3 g厚朴酚/kg商品饲料）显着增加了金鱼肝脏中抗炎细胞因子（IL-10、TGF-β和IL-4）的表达水平（p < .05）。此外，促炎细胞因子（IL-1β、IL-8和IFN-γ）的表达水平没有显着增加。随后，本研究调查了金鱼喂食厚朴酚后对嗜水气单胞菌和维罗尼单胞菌感染的抵抗力。结果表明，每天饲喂 3 g 厚朴酚/kg 商业饲料的治疗组在嗜水气单胞菌和维罗尼气单胞菌 (p = .0351) 感染 10 天后，存活率分别增加了 23.1% 和 38.5%。同时，饲喂厚朴酚30天后，金鱼的生长性能（体重、体长）、主要内脏器官（肝、脾、肾、肠）及血清生化指标（ATL、AST）均未出现明显不良影响。治疗组中 TP 浓度不断增加 (p < .05)。应激反应mRNA表达结果表明，经过30天处理后，cyp1a和hsp70的表达水平显着下调（p < .05），并且在商业饲料日粮后这两个基因恢复到与对照组相似的水平。简而言之，添加厚朴酚的饮食通过增强其抗炎能力，保护宿主免受嗜水气单胞菌和维罗尼单胞菌引起的过度免疫反应，并且对喂养没有不良影响。
Aeromonas hydrophila and A. veronii are widespread and important critical pathogenic bacteria in the aquaculture industry and cause severe economic damage. At present, magnolol has been proved to be a broad-spectrum antibacterial activity, such as A. hydrophila, Staphylococcus aureus and Streptococcus mutans. In order to explore the cause of in vivo disease resistance of magnolol and promote its safe application in aquaculture, the pathological detection and changes in immune indicators of fish after feeding with magnolol were conducted in this paper. Results showed that the diets supplemented with magnolol (3 g magnolol/kg commercial feed) significantly increase the expression level of anti-inflammatory cytokines (IL-10, TGF-β and IL-4) in the liver of goldfish (p < .05). Additionally, the expression levels of proinflammatory cytokines (IL-1β, IL-8 and IFN-γ) did not increase significantly. Subsequently, this study investigated the resistance of goldfish to A. hydrophila and A. veronii infection after feeding with magnolol. The results showed that the survival rates of treatment groups fed 3 g magnolol/kg commercial feed daily increased by 23.1% and 38.5% after 10 days post A. hydrophila and A. veronii (p = .0351) infection, respectively. Meanwhile, growth performance (body weight and length), major internal organs (liver, spleen, kidney and intestine) and the serum biochemistry indicators (ATL and AST) all exhibited no significant adverse effects after the goldfish fed with magnolol for 30 days. TP showed an increasing concentration in the treatment group (p < .05). Results of the mRNA expression of stress response indicated that the expression level of cyp1a and hsp70 was significantly down-regulated after a 30-day treatment (p < .05), and the two genes recovered to the similar level as the control group after a commercial feed diet. In brief, the diets supplemented with magnolol protected the host from the excessive immune response caused by A. hydrophila and A. veronii via enhancing its anti-inflammatory capacity and had no adverse effects with feeding.