Surface antigens of Biomphalaria glabrata (Gastropoda) hemocytes: functional heterogeneity in cell subpopulations recognized by a monoclonal antibody.
Surface antigens of Biomphalaria glabrata (Gastropoda) hemocytes: functional heterogeneity in cell subpopulations recognized by a monoclonal antibody.
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DOI：
10.1016/0022-2011(85)90007-2
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发表时间：
1985-03
影响因子：
3.4
通讯作者：
T. Yoshino;W. O. Granath
中科院分区：
文献类型：
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作者：
T. Yoshino;W. O. Granath
用体细胞融合方法产生的血细胞特异性单抗(MAb)鉴定了血细胞表膜标志物(BGH1)。BGH1表位是在两个品系的蜗牛的循环中的玻璃贴壁血细胞亚群上表达的。来自PR白化(M系)B的循环血细胞中大约40%是BGH1−，而在10-R2钉螺品系中BGH1+细胞的流行率为10%。当血细胞牢固地附着在玻璃表面并铺展在玻璃表面时，BGH1+细胞在形态上与BGH1−细胞不同，因为它们的卵圆形和沿外质缘存在的短而薄的丝状突起。相比之下，BGH1−血细胞具有更多的多形性，并具有长的棘状丝状足基。此外，BGH1表位是胰酶抗性的，经多聚甲醛或多聚甲醛/甲醇固定后，仍能与探针单抗发生抗原反应。然而，戊二醛的固定显著减少了单抗与BGH1表面表位的结合。由于幼年(1-2 mm)到成年(10-12 mm)钉螺的循环血细胞群同时由BGH1+和BGH1−亚群组成，因此BGH1决定簇没有明显的年龄相关性表达。携带表位的血细胞在最小的钉螺大小类(1-2毫米)和较大的钉螺(3-4和10-12毫米)之间的流行程度的定量变化被认为是由于BGH1−血细胞在血液循环中的不同产生和/或释放，而不是单个细胞表面抗原表达的随年龄的逐渐变化。旨在评估单抗反应血细胞的体外吞噬能力和溶酶体酸性磷酸酶(APase)活性的实验表明，与缺乏表面标志的BGH1+细胞相比，BGH1+细胞的吞噬能力和APase产生活性均显著降低。自从PR白化病菌株OB。与10-R2品系的钉螺相比，PR白化钉螺拥有更高比例的BGH1−血细胞和更低的循环细胞总浓度，因此它们对外来物质发动细胞反应的自然能力可能会降低。
A hemocyte surface membrane marker (BGH1) has been identified using hemocyte-specific monoclonal antibodies (mABs) generated by somatic cell fusion methods. The BGH1epitope was expressed on a subpopulation of circulating, glass-adherent blood cells from two strains of the snail,Biomphalaria glabrata. Approximately 40% of the circulating hemocytes from the PR albino (M-line)B. glabratastrain were BGH1−, compared to a prevalence of 10% BGH1+cells in the 10-R2 snail strain. When hemocytes were firmly attached and spread on a glass surface, BGH1+cells were morphologically distinguishable from BGH1−cells by their ovoid shape and the presence of short, thin filopodial projections along the ectoplasmic border. In contrast, BGH1−hemocytes were more pleomorphic and possessed long, spike-like filopodia. Moreover, the BGH1epitope was trypsin-resistant and retained its antigenic reactivity with probe mABs following fixation with paraformaldehyde or paraformaldehyde/MeOH. Fixation with glutaraldehyde, however, significantly reduced mAB binding to the BGH1surface epitope. There was no apparent age-dependent expression of the BGH1determinant since circulating hemocyte populations in very young (1–2 mm) to adult (10–12 mm) snails were composed of both BGH1+and BGH1−subpopulations. Quantitative shifts in the prevalence of epitope-bearing hemocytes between the smallest snail size class (1–2 mm) and the larger snails (3–4 and 10–12 mm) are believed to be due to a differential production and/or release of BGH1−hemocytes within the blood circulation rather than a gradual age-related change in the expression of surface antigens on individual cells. Experiments designed to assess the in vitro phagocytic capability and lysosomal acid phosphatase (APase) activity of mAB-reactive hemocytes revealed that BGH1+cells, when compared to those lacking the surface marker, were significantly reduced in both their phagocytic and APase-producing activities. Since the PR albino strain ofB. glabratapossesses a higher proportion of BGH1−hemocytes and a lower total concentration of circulating cells than do snails of the 10-R2 strain, PR albino snails are thus potentially reduced in their natural capacity to mount cellular reactions against foreign materials.