Immunohistochemical analysis of a muscle ankyrin-repeat protein, Arpp, in paraffin-embedded tumors: evaluation of Arpp as a tumor marker for rhabdomyosarcoma
Immunohistochemical analysis of a muscle ankyrin-repeat protein, Arpp, in paraffin-embedded tumors: evaluation of Arpp as a tumor marker for rhabdomyosarcoma
复制标题
DOI：
10.1016/j.humpath.2005.04.014
复制
发表时间：
2005-06-01
期刊：
影响因子：
3.3
通讯作者：
Yoshida, H
中科院分区：
文献类型：
--
作者：
Ishiguro, N;Motoi, T;Yoshida, H
Arpp是一种包含锚定重复序列、PEST基序和富含脯氨酸区域的蛋白质，是最近鉴定的仅在横纹肌中表达的蛋白质。本研究综合分析了其在不同组织学类型软组织肉瘤中的表达，并评估其在横纹肌肉瘤（RMS）鉴别诊断中的潜在应用。福尔马林固定，石蜡包埋的组织，包括37例RMS病例，88例非RMS肉瘤，和38例癌，分析了Arpp表达。37例RMS中33例（89.2%）ARPP阳性。Western blot分析显示，在所有的RMS测试情况下的表达Arpp。Arpp的高表达通常与肿瘤细胞骨骼肌分化的形态学证据相关。而在非RMS肿瘤中，Arpp的阳性率为6.3%（8/126）。在恶性纤维组织细胞瘤（2/27）、滑膜肉瘤（1/11）、尤文肉瘤（115）和上皮样肉瘤（3/5）中观察到局灶性或弱表达，而1例上皮样肉瘤显示Arpp强表达。与肌源性标志物的比较分析显示，Arpp的敏感性（89.2%）高于肌红蛋白（59.6%），与肌生成素（88.2%）、MyoD（80.6%）、肌肉特异性肌动蛋白（83.8%）和结蛋白（89.2%）相当。这些结果表明，Arpp是敏感和特异性RMS。因此，我们认为Arpp是一种新的骨骼肌特异性标志物，可用于RMS的鉴别诊断。(c)2005年爱思唯尔公司All rights reserved.
Arpp, a protein including an ankyrin-repeat, PEST motif, and proline-rich region, is a recently identified protein that is exclusively expressed in striated muscles. This study comprehensively analyzed its expression among soft tissue sarcomas of various histological types and evaluated its potential use for the differential diagnosis of rhabdomyosarcoma (RMS). Formalin-fixed, paraffin-embedded tissues, including 37 RMS cases, 88 non-RMS sarcomas, and 38 carcinomas, were analyzed for Arpp expression. Arpp was detected in 33 (89.2%) of 37 RMS cases by immunohistochemistry. Western blot analysis revealed expression of Arpp in all RMS cases tested. High expression of Arpp was generally associated with morphological evidence of skeletal muscle differentiation of tumor cells. In contrast, Arpp displayed 6.3% (8/126) positivity among the non-RMS tumors. Focal or weak expression was seen in malignant fibrous histiocytoma (2/27), synovial sarcoma (1/11), Ewing sarcoma (115), and epithelioid sarcoma (3/5), whereas one epithelioid sarcoma displayed strong expression for Arpp. A comparative analysis of the Arpp profile with that of myogenic markers in RMS revealed that the sensitivity of Arpp (89.2%) was higher than that of myoglobin (59.6%) and comparable with that of myogenin (88.2%), MyoD (80.6%), muscle-specific actin (83.8%), and desmin (89.2%). These results suggested that Arpp is sensitive to and specific for RMS. Thus, we proposed that Arpp is a novel skeletal muscle specific marker, which is useful for differential diagnosis of RMS. (c) 2005 Elsevier Inc. All rights reserved.