Immune responses to porphyromonas gingivalis infection suppress systemic inflammatory response in experimental murine model.
Immune responses to porphyromonas gingivalis infection suppress systemic inflammatory response in experimental murine model.
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发表时间：
2011-04
影响因子：
3.2
通讯作者：
Koji Naruishi;K. Omori;Hiroshi Maeda;Norihiro Sonoi;K. Funakoshi;Kimito Hirai;M. Ishii;K. Kubo;H. Kobayashi;T. Tomiyama;Daisuke Yamamoto;I. Tanimoto;Kazushi Kunimatsu;S. Takashiba
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作者：
Koji Naruishi;K. Omori;Hiroshi Maeda;Norihiro Sonoi;K. Funakoshi;Kimito Hirai;M. Ishii;K. Kubo;H. Kobayashi;T. Tomiyama;Daisuke Yamamoto;I. Tanimoto;Kazushi Kunimatsu;S. Takashiba
牙周炎是由牙龈卟啉单胞菌（Porphyromonas gingivalis，P. gingivalis）等牙周致病菌引起的局部感染性疾病，其严重程度与机体免疫反应密切相关。近年来，由于口腔病原体对循环系统的侵袭增加，牙周炎与全身性疾病如糖尿病和动脉粥样硬化的发展有关。然而，局部和全身感染反应之间的关联仍不清楚。在本研究中，我们研究了有或没有细菌感染的动物的生物反应的差异。用活的牙龈卟啉单胞菌W83皮下感染Balb/c小鼠后，根据实验方案收集血清、皮肤和肝脏。采用苏木精-伊红染色法观察皮肤和肝组织病理学变化，ELISA法检测血清IL-6水平。在整个实验期间，连续观察小鼠的状况。正如预期的那样，在炎症皮肤处观察到严重的白细胞浸润，对应于细菌挑战的数量。虽然没有观察到皮肤的炎症外观，但在没有细菌攻击的小鼠中，血清IL-6水平显著增加（P <0.01，Student’s t-检验）并且肝组织受损。有趣的是，虽然观察到皮肤的严重炎症外观，但血清IL-6水平没有增加，并且在3次细菌攻击组的肝脏中没有观察到炎症反应。重要的是，在3次细菌攻击的小鼠血液中检测到针对牙龈卟啉单胞菌W83的免疫球蛋白G，这对应于体重减轻和存活的改善。总之，虽然多重感染发展为严重的局部炎症，但免疫系统应足以保护全身炎症反应。
Periodontitis is a localized infectious disease caused by periodontopathic bacteria such as Porphyromonas gingivalis (P. gingivalis), and the severity correlates to significance of immune responses. Recently, it has been reported that periodontitis is associated with the development of systemic disease such as diabetes and atherosclerosis because of increasing invasion of oral pathogens to the circulation. However, the association between local and systemic infectious responses is still unclear. In the present study, we examined the differences of biological responses in animals with or without bacterial infection. After Balb/c mice were infected subcutaneously with live P. gingivalis W83, serum, skin and liver were collected according to experimental protocol. The skin and liver tissues were observed pathologically by haematoxylin-eosin staining, and serum IL-6 levels were measured using ELISA method. Throughout the experimental period, conditions of the mice were observed continuously. As expected, severe infiltration of leukocytes were observed at inflamed skin corresponding to the number of bacterial challenges. Although no inflammatory appearance of skin was observed, serum IL-6 levels were increased dramatically (P <0.01, Student's t-test) and liver tissues were injured in the mice without bacterial challenge. Interestingly, although severe inflammatory appearance of the skin was observed, serum IL-6 levels were not increased and no inflammatory responses were observed in the liver of the 3-times bacterially challenged group. Importantly, immunoglobulin G against P. gingivalis W83 was detected in the blood of mice with 3-times bacterial challenge corresponding to improvement of weight loss and survival. In conclusion, although multiple infections develop severe localized inflammation, the immune system should be sufficient to protect the systemic inflammatory responses.