Efficient Production of Optically Pure D-Lactic Acid from Raw Corn Starch by Using a Genetically Modified L-Lactate Dehydrogenase Gene-Deficient and α-Amylase-Secreting Lactobacillus plantarum Strain
Efficient Production of Optically Pure D-Lactic Acid from Raw Corn Starch by Using a Genetically Modified L-Lactate Dehydrogenase Gene-Deficient and α-Amylase-Secreting Lactobacillus plantarum Strain
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DOI：
10.1128/aem.01514-08
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发表时间：
2009-01-15
影响因子：
4.4
通讯作者：
Kondo, Akihiko
中科院分区：
文献类型：
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作者：
Okano, Kenji;Zhang, Qiao;Kondo, Akihiko
为了实现玉米淀粉直接高效发酵生产光学纯D-乳酸，我们构建了缺失乳酸脱氢酶基因(LDHL1)的植物乳杆菌，并引入了编码牛链球菌148α-淀粉酶(AMYA)的表达载体。得到的菌株只从葡萄糖中产生D-乳酸，并成功地表达了AMYA。以玉米淀粉为原料，通过分泌淀粉酶，实现了D-乳酸的直接发酵。发酵48h，乳酸产量为73.2g/L，糖得率为0.85g/g，光学纯度为99.6%。此外，还构建了一株将ldhL1基因替换为AMYA分泌型表达框的菌株。利用该菌株，在没有抗生素选择压力的情况下，实现了以玉米淀粉为原料直接发酵D-乳酸。这是首次报道以生淀粉为原料直接发酵D-乳酸。
In order to achieve direct and efficient fermentation of optically pure D-lactic acid from raw corn starch, we constructed L-lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum and introduced a plasmid encoding Streptococcus bovis 148 alpha-amylase (AmyA). The resulting strain produced only D-lactic acid from glucose and successfully expressed amyA. With the aid of secreting AmyA, direct D-lactic acid fermentation from raw corn starch was accomplished. After 48 h of fermentation, 73.2 g/liter of lactic acid was produced with a high yield (0.85 g per g of consumed sugar) and an optical purity of 99.6%. Moreover, a strain replacing the ldhL1 gene with an amyA-secreting expression cassette was constructed. Using this strain, direct D-lactic acid fermentation from raw corn starch was accomplished in the absence of selective pressure by antibiotics. This is the first report of direct D-lactic acid fermentation from raw starch.