Synergistic effect of insulin and follicle-stimulating hormone on biochemical and morphological differentiation of porcine granulosa cells in vitro.
Synergistic effect of insulin and follicle-stimulating hormone on biochemical and morphological differentiation of porcine granulosa cells in vitro.
复制标题
胰岛素和卵泡刺激素对猪颗粒细胞体外生化和形态分化的协同作用。
DOI：
10.1095/biolreprod39.2.379
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发表时间：
1988
影响因子：
3.6
通讯作者：
Schomberg,DW
中科院分区：
文献类型：
--
作者：
Amsterdam,A;May,JV;Schomberg,DW
胰岛素和卵泡刺激素（FSH）已被证明可以促进颗粒细胞的体外分化。为了深入了解这一过程，我们评估了这些激素单独和联合使用的效果，结果：（1）在体外培养的小窦卵泡颗粒细胞中，LH/hCG受体的诱导和孕酮的分泌与颗粒细胞的超微结构和LH/hCG受体的分布密切相关。（对照组）在没有外源激素的情况下培养，显示出很少的微绒毛和间隙连接;线粒体、内质网和高尔基复合体都发育不良。孕酮分泌可忽略不计，细胞结合少量[125 I]碘-hCG。胰岛素处理增加了间隙连接的形成，以及平滑和粗糙内质网和高尔基复合体发育的程度（所有p < 0.05），但不影响线粒体超微结构或体积。相对于对照组，胰岛素治疗适度但显著增加[125 I]碘-hCG结合和孕酮分泌（p < 0.001）。FSH处理对细胞超微结构的影响与胰岛素相似，并促进线粒体和滑面内质网的发育以及微绒毛的形成（P < 0.05）。与胰岛素处理的样品相比，FSH显著增加[125 I]-hCG结合和孕酮分泌（p < 0.001）。与单独使用任一激素处理相比，胰岛素和FSH联合处理显著增加缝隙连接和微绒毛形成，并促进滑面内质网和高尔基复合体的发育（p < 0.05）。此外，联合治疗产生了更大的线粒体与管状christae。与形态发育一致，胰岛素和FSH联合治疗显著增加孕酮分泌和[125 I]碘-hCG结合（p < 0.001）。放射自显影分析表明，聚集细胞一般表现出更高的LH/hCG受体密度比非聚集细胞，并显着更高的整体受体密度相比，非聚集细胞或细胞与胰岛素或FSH单独处理。我们的研究结果表明，胰岛素和FSH促进形态分化的颗粒细胞在协同的方式，刺激缝隙连接和微绒毛的形成，增强线粒体、内质网和高尔基体的发育。这些激素介导的形态发育与它们对这些细胞中生化过程的影响是一致的
Insulin and follicle-stimulating hormone (FSH) have been shown to facilitate granulosa cell differentiation in vitro. To gain insight into this process, we evaluated the effects of these hormones, alone and in combination, upon the biochemical parameters of luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor induction and progesterone secretion concomitantly with morphometric analysis of granulosa cell ultrastructure and LH/hCG receptor distribution by quantitative autoradiography under light microscopy.Granulosa cells isolated from small antral follicles (controls) cultured in the absence of exogenous hormones exhibited few microvilli and gap junctions; the mitochondria, endoplasmic reticulum, and Golgi complex were all poorly developed. Progesterone secretion was negligible and the cells bound little [125I]iodo-hCG. Insulin treatment increased gap junction formation, and the extent of smooth and rough endoplasmic reticulum and Golgi complex development (all p < 0.05) but did not affect mitochondrial ultrastructure or volume. Insulin treatment modestly but significantly increased [125I]iodo-hCG binding and progesterone secretion relative to controls (p < 0.001). FSH treatment had a similar effect to insulin on cell ultrastructure and additionally enhanced development of the mitochondria and smooth endoplasmic reticulum as well as formation of the microvilli (p < 0.05). FSH significantly increased [125I]iodo-hCG binding and progesterone secretion relative to insulin-treated samples (p < 0.001).Combined treatment with insulin and FSH markedly increased gap junction and microvilli formation and enhanced the development of the smooth endoplasmic reticulum and the Golgi complex relative to treatment with either hormone alone (p < 0.05). Additionally, the combined treatment produced larger mitochondria with tubular christae. Consistent with the morphological development, the combined treatment of insulin and FSH significantly increased progesterone secretion and [125I]iodo-hCG binding (p < 0.001). Autoradiographic analysis showed that aggregated cells in general exhibited higher LH/hCG receptor density than nonaggregated cells, and a significantly higher overall receptor density compared to nonaggregated cells or to cells treated either with insulin or FSH alone.Our results indicate that insulin and FSH facilitate morphological differentiation of the granulosa cell in a synergistic manner, stimulating gap junctions and microvilli formation and enhancing development of the mitochondria, endoplasmic reticulum, and Golgi complex. The morphologic development mediated by these hormones is consistent with their effects upon biochemical processes in these cells