The leader peptide of yeast gene CPA1 is essential for the translational repression of its expression
The leader peptide of yeast gene CPA1 is essential for the translational repression of its expression
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DOI：
10.1016/0092-8674(87)90618-0
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发表时间：
1987-06
期刊：
影响因子：
64.5
通讯作者：
M. Werner;A. Feller;F. Messenguy;A. Piérard
中科院分区：
文献类型：
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作者：
M. Werner;A. Feller;F. Messenguy;A. Piérard
编码精氨酸途径氨甲酰磷酸合成酶谷氨酰胺酶亚单位的基因cpm在转录后水平被精氨酸抑制。CPAF基因5‘端含有一个25密码子的上游开放阅读框。这一特征对于抑制CPA7表达的重要性已经通过寡核苷酸定向突变和体内获得的结构性和显性突变的测序进行了分析。结果表明，上游开放阅读框的产物先导肽在精氨酸特异性抑制CPA7的过程中起着重要的负性作用。提出了一个CPA7的翻译调控模型，该模型考虑了影响前导肽的突变的顺式显性。
The expression of gene CPM, encoding the glutaminase subunit of the arginine pathway carbamoyl-phosphate synthetase, is repressed by arginine at a posttranscriptional level. The 5’region of CPAf mRNA contains a 25 codon upstream open reading frame. The importance of this feature for the repression of CPA7 expression has been analyzed by oligonucleotide-directed mutagenesis and by sequencing of constitutive &-dominant mutations obtained in vivo. The results show that the leader peptide, the product of the upstream open reading frame, plays an essential, negative role in the specific repression of CPA7 by arginine. A model of translational regulation of CPA7 is proposed that takes into account the cis-dominance of the mutations affecting the leader peptide.