Synchrotron-based in vivo tracking of implanted mammalian cells
Synchrotron-based in vivo tracking of implanted mammalian cells
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DOI：
10.1016/j.ejrad.2008.04.058
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发表时间：
2008-12-01
影响因子：
3.3
通讯作者：
Juurlink, B. H. J.
中科院分区：
文献类型：
--
作者：
Hall, C. J.;Schultke, E.;Juurlink, B. H. J.
我们已经开发了一种X射线成像协议，允许大量组织内少量植入细胞的3D可视化。使用惰性金纳米颗粒的自然内吞作用标记细胞。由此产生的电子密度的局部增加允许从这些标记细胞的小簇获得高成像对比度。使用这种技术，我们已经成像C6胶质瘤细胞内的模型动物的大脑。通过将细胞暴露于掺入生长培养基中的胶体金来标记细胞。将负载金的胶质瘤细胞植入成年雄性Wistar大鼠的脑中。在肿瘤发展2周后，处死动物，并在意大利Elettra同步加速器上的SYRMEP成像站获得完整颅骨的图像。使用混合吸收和相衬技术在24 keV的X射线能量下进行计算机断层扫描。在所得到的体积数据集中，肿瘤体积清晰可见，恶性生长的浸润性质得到了很好的证明。虽然该方案是使用这种特定的恶性脑肿瘤模型开发的，但据信它将有可能与其他细胞系一起使用。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
We have developed an X-ray imaging protocol that permits 3D visualisation of a small number of implanted cells within bulk tissue. The cells are marked using natural endocytosis of inert gold nano-particles. The resulting local increase in electron density allows high imaging contrast to be obtained from small clusters of these marked cells. Using this technique we have imaged C6 glioma cells within the brain of a model animal. The cells were marked by exposing them to colloidal gold incorporated in the growth media. Gold-loaded glioma cells were implanted into the brains of adult male Wistar rats. After tumours had been allowed to develop for up to 2 weeks, the animals were sacrificed and images of the intact cranium were acquired at the SYRMEP imaging station on the Elettra synchrotron in Italy. Computed tomography was performed using mixed absorption and phase contrast techniques at an X-ray energy of 24 keV. In the resulting volume datasets the tumour bulk is clearly visible and the infiltrating nature of the malignant growth is well demonstrated. Although the protocol was developed Using this particular model of malignant brain tumour, it is believed that it will be possible to use it with other cell lines. (C) 2008 Elsevier Ireland Ltd. All rights reserved.