Synergy of ultrasound microbubbles and vancomycin against Staphylococcus epidermidis biofilm
Synergy of ultrasound microbubbles and vancomycin against Staphylococcus epidermidis biofilm
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超声微泡与万古霉素对抗表皮葡萄球菌生物膜的协同作用
DOI：
10.1093/jac/dks490
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发表时间：
2013-04-01
影响因子：
5.2
通讯作者：
Yu, Jialin
中科院分区：
文献类型：
--
作者：
Dong, Ying;Chen, Shaojie;Yu, Jialin
目的：主要由表皮葡萄球菌引起的器械相关生物膜感染难以用常规抗生素有效治疗。本研究旨在探讨超声介导微泡联合万古霉素抗生物膜的作用及其机制。在OptiCellTM室中建立表皮生物膜以促进超声暴露。制备微泡并稀释至1%和4%（v/v）的浓度。以300 kHz和0.5 W/cm（2）的频率施加超声5 min，占空比为50%。在预先形成的生物膜上使用峰值血清浓度为32 mg/L的万古霉素24 h。对生物膜进行抗生素敏感性试验，以确认超声和万古霉素之间的协同作用。将暴露于超声介导的微泡与万古霉素组合的生物膜进行平板计数和显微镜检查。结果：超声和超声介导的微泡均能增强生物膜对万古霉素的敏感性。不含万古霉素的超声介导的微泡可以对生物膜产生杀菌作用。还观察到气泡剂量依赖性生物效应。在万古霉素存在的情况下，暴露于超声介导的微泡的生物膜显示出比其他处理组显著更多的微孔和更多的生物膜厚度减少（P
Objectives: Device-associated biofilm infections primarily caused by Staphylococcus epidermidis are difficult to treat effectively with conventional antibiotics. The aim of this study was to investigate the anti-biofilm effect of ultrasound-mediated microbubbles combined with vancomycin and to explore underlying mechanisms.Methods: Twenty-four hour S. epidermidis biofilms were established in OptiCell (TM) chambers to facilitate ultrasound exposure. Microbubbles were prepared and diluted to concentrations of 1% and 4% (v/v). Ultrasound was applied for 5 min at 300 kHz and 0.5 W/cm(2), with a 50% duty cycle. Vancomycin at the peak serum concentration of 32 mg/L was used on preformed biofilms for 24 h. Antibiotic susceptibility tests were conducted on biofilms to confirm the synergy between ultrasound and vancomycin. Biofilms exposed to ultrasound-mediated microbubbles combined with vancomycin were subjected to plate counting and microscopic examinations. A vancomycin penetration test was also performed.Results: Ultrasound and ultrasound-mediated microbubbles both enhanced biofilm susceptibility to vancomycin. Ultrasound-mediated microbubbles without vancomycin could exert a bactericidal effect on biofilms. A bubble dose-dependent bioeffect was also observed. In the presence of vancomycin, biofilms exposed to ultrasound-mediated microbubbles exhibited significantly more micropores and more reduction in biofilm thickness than other treatment groups (P