Measurement of phenol and p-cresol in urine and feces using vacuum microdistillation and high-performance liquid chromatography
Measurement of phenol and p-cresol in urine and feces using vacuum microdistillation and high-performance liquid chromatography
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DOI：
10.1016/j.ab.2008.09.034
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发表时间：
2009-01-01
影响因子：
2.9
通讯作者：
Bird, Anthony R.
中科院分区：
文献类型：
--
作者：
King, Roger A.;May, Bruce L.;Bird, Anthony R.
在这篇文章中，我们描述了一种简单、灵敏、准确和可重复的方法来测量人类尿液和粪便中的苯酚和对甲酚（4-甲基苯酚）。我们检查了一些参数，以确定所有的最佳提取方案。采用低温真空微蒸馏对样品萃取物进行纯化。采用高效液相色谱（HPLC）分离，分离时间约为15 min，荧光定量波长为284/310 nm。尿液中苯酚的检出限为2 ng/ml，粪便中苯酚的检出限为20 ng/g，对甲酚的检出限为10 ng/ml，粪便中甲酚的检出限为100 ng/g。为了比较，尿液中苯酚的近似平均值为3 μ g/ml，对甲酚的近似平均值为30 μ g/ml，粪便中苯酚的近似平均值为1 μ g/g，对甲酚的近似平均值为50 μ g/g。一个有经验的分析人员每天可以使用这种方法处理60个样品。英国皇家版权所有(C) 2008 Elsevier Inc.出版版权所有。
In this article, we describe a simple, sensitive, accurate, and repeatable method for the measurement of phenol and p-cresol (4-methylphenol) in human urine and feces. We examined a number of parameters to identify all optimal extraction protocol. Purification of sample extracts was achieved by low-temperature vacuum microdistillation. Separation was achieved in approximately 15 min by high-performance liquid chromatography (HPLC) with quantification by fluorescence at 284/310 nm. Limits of detection for phenol were 2 ng/ml for urine and 20 ng/g for feces, and those for p-cresol were 10 ng/ml for urine and 100 ng/g for feces. For comparison, approximate mean values for urine are 3 mu g/ml for phenol and 30 mu g/ml for p-cresol, and those for feces are 1 mu g/g for phenol and 50 mu g/g for p-cresol. An experienced analyst can process 60 samples each day using this method. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.