The murine atrial myosin light chain-2 gene: a member of an evolutionarily conserved family of contractile proteins
The murine atrial myosin light chain-2 gene: a member of an evolutionarily conserved family of contractile proteins
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DOI：
10.1159/000056782
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发表时间：
2000-01-01
期刊：
影响因子：
--
通讯作者：
van Bilsen, M
中科院分区：
文献类型：
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作者：
Doevendans, PA;Bronsaer, R;van Bilsen, M
最近，近完整的调控心房肌球蛋白轻链（MLC-2a）的cDNA被克隆。心房特异性亚型已被证明是心腔特化的有用分子标记物。因此，该基因的调控序列将为心肌细胞分化和心房特异性转录调控提供线索。在这里，我们报告的MLC-2a基因（Mylc 2a）的小鼠基因组序列的鉴定。鉴定整个5'侧翼区并测序。此外，还测定了外显子-内含子边界和3'侧翼区。序列比较显示，小鼠葡萄糖激酶基因的最后外显子（11）存在于染色体11上，Mylc 2a转录起始位点上游2.0 kb处。此外，我们将该基因的结构与其他肌球蛋白轻链进行了比较，以显示进化保守性。内含子-外显子边界高度保守，在更复杂的哺乳动物物种中发现内含子大小增加。在氨基酸水平上，人和小鼠MLC-2a序列之间有95%的同源性。版权所有（C）2000 S. Karger AG，巴塞尔。
Recently the near complete cDNA of the regulatory atrial myosin light chain (MLC-2a) was cloned. The atrial specific isoform has been shown to be a useful molecular marker for cardiac chamber specification. Therefore, the regulatory sequence of the gene will provide clues on cardiomyocyte differentiation and atrial specific transcription regulation. Here we report the identification of the murine genomic sequence of the MLC-2a gene (Mylc2a). The entire 5' flanking region was identified and sequenced. In addition, the exon-intron boundaries and 3' flanking region were determined. Sequence comparison revealed the presence of the final exon (11) of the mouse glucokinase gene on chromosome 11, 2.0 kb upstream of the Mylc2a transcription start site. In addition, we compared the structure of the gene to other myosin light chains to show evolutionary conservation. The intron-exon boundaries turned out to be highly conserved and an increasing intron size in more complex mammalian species was found. At the amino acid level there is 95 % homology between the human and mouse MLC-2a sequence. Copyright (C) 2000 S. Karger AG, Basel.