Reductase and Light Programmatical Gated DNA Nanodevice for Spatiotemporally Controlled Imaging of Biomolecules in Subcellular Organelles under Hypoxic Conditions
Reductase and Light Programmatical Gated DNA Nanodevice for Spatiotemporally Controlled Imaging of Biomolecules in Subcellular Organelles under Hypoxic Conditions
复制标题
还原酶和光程序门控 DNA 纳米装置用于低氧条件下亚细胞细胞器中生物分子的时空控制成像
DOI：
10.1021/acsami.1c08979
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发表时间：
2021
影响因子：
9.5
通讯作者：
Yang Ronghua
中科院分区：
文献类型：
--
作者：
Liu Jin;Yang Le;Xue Caoye;Huang Ge;Chen Shiya;Zheng Jing;Yang Ronghua
监测与缺氧相关的亚细胞细胞器的变化将有助于我们更深入地了解与缺氧相关的代谢途径，进一步帮助我们在亚细胞水平上认识各种疾病。然而，目前还缺乏实时、原位、可控的低氧条件下亚细胞细胞器的生物传感手段。在这里，我们报道了一种还原酶和光编程门控纳米器件，将光响应DNA探针集成到低氧响应的金属-有机骨架中，用于在低氧条件下对亚细胞细胞器中的生物分子进行时空控制成像。一种小分子修饰策略被应用于赋予纳米设备靶向亚细胞细胞器的能力。以线粒体三磷酸腺苷在低氧条件下的动态变化为模型生理过程。该方法在从小鼠模型中获得的活细胞和肿瘤组织切片中得到验证。由于生物分子的高度集成性、易获得性以及对活细胞和组织的可获得性，我们设想该概念和方法可以进一步扩展到通过时空可控的方法来监测低氧条件下其他亚细胞细胞器中的生物分子。
Monitoring hypoxia-related changes in subcellular organelles would provide deeper insights into hypoxia-related metabolic pathways, further helping us to recognize various diseases on subcellular level. However, there is still a lack of real-time,in situ, and controllable means for biosensing in subcellular organelles under hypoxic conditions. Herein, we report a reductase and light programmatical gated nanodeviceviaintegrating light-responsive DNA probes into a hypoxia-responsive metal–organic framework for spatiotemporally controlled imaging of biomolecules in subcellular organelles under hypoxic conditions. A small-molecule-decorated strategy was applied to endow the nanodevice with the ability to target subcellular organelles. Dynamic changes of mitochondrial adenosine triphosphate under hypoxic conditions were chosen as a model physiological process. The assay was validated in living cells and tumor tissue slices obtained from mice models. Due to the highly integrated, easily accessible, and available for living cells and tissues, we envision that the concept and methodology can be further extended to monitor biomolecules in other subcellular organelles under hypoxic conditions with a spatiotemporal controllable approach.