Verticillium dahliae manipulates plant immunity by glycoside hydrolase 12 proteins in conjunction with carbohydrate-binding module 1
Verticillium dahliae manipulates plant immunity by glycoside hydrolase 12 proteins in conjunction with carbohydrate-binding module 1
复制标题
大丽黄萎病通过糖苷水解酶 12 蛋白与碳水化合物结合模块 1 结合来操纵植物免疫
DOI：
10.1111/1462-2920.13695
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发表时间：
2017-05-01
影响因子：
5.1
通讯作者：
Dai, Xiao-Feng
中科院分区：
文献类型：
--
作者：
Gui, Yue-Jing;Chen, Jie-Yin;Dai, Xiao-Feng
糖苷水解酶12（GH12）蛋白在卵菌中作为毒力因子和病原相关分子模式（PAMPs）发挥作用。然而，真菌GH12蛋白的致病机制尚未被表征。在这项研究中，我们证明了由真菌大丽轮枝菌Vd991产生的六种GH12蛋白中的两种，VdEG1和VdEG3作为PAMP触发细胞死亡和PAMP触发的免疫（PTI），独立于它们在本氏烟草中的酶活性。一个63个氨基酸的肽的VdEG3是足够的细胞死亡诱导活性，但这是不是为相应的肽的VdEG1的情况。进一步的研究表明，VdEG1和VdEG3以不同的方式触发PTI：BAK 1是VdEG1和VdEG3触发免疫所必需的，而SOBIR 1是VdEG1触发免疫所特异性需要的。本萨米亚纳与卵菌不同，卵菌利用RXLR效应子抑制宿主免疫，碳水化合物结合模块家族1（CBM 1）蛋白结构域抑制GH12蛋白诱导的细胞死亡。此外，在感染N.本塞姆氏菌和棉花中，VdEG1和VdEG3分别作为PAMPs和毒力因子，指示宿主依赖的分子功能。这些结果表明，VdEG1和VdEG3与BAK1和SOBIR1受体样激酶不同地结合以触发N. benthamiana，并与含有CBM 1的蛋白质一起操纵植物免疫。
Glycoside hydrolase 12 (GH12) proteins act as virulence factors and pathogen-associated molecular patterns (PAMPs) in oomycetes. However, the pathogenic mechanisms of fungal GH12 proteins have not been characterized. In this study, we demonstrated that two of the six GH12 proteins produced by the fungus Verticillium dahliae Vd991, VdEG1 and VdEG3 acted as PAMPs to trigger cell death and PAMP-triggered immunity (PTI) independent of their enzymatic activity in Nicotiana benthamiana. A 63-amino-acid peptide of VdEG3 was sufficient for cell death-inducing activity, but this was not the case for the corresponding peptide of VdEG1. Further study indicated that VdEG1 and VdEG3 trigger PTI in different ways: BAK1 is required for VdEG1- and VdEG3-triggered immunity, while SOBIR1 is specifically required for VdEG1-triggered immunity in N. benthamiana. Unlike oomycetes, which employ RXLR effectors to suppress host immunity, a carbohydrate-binding module family 1 (CBM1) protein domain suppressed GH12 protein-induced cell death. Furthermore, during infection of N. benthamiana and cotton, VdEG1 and VdEG3 acted as PAMPs and virulence factors, respectively indicative of host-dependent molecular functions. These results suggest that VdEG1 and VdEG3 associate differently with BAK1 and SOBIR1 receptor-like kinases to trigger immunity in N. benthamiana, and together with CBM1-containing proteins manipulate plant immunity.