Chick homeobox gene cDlx expression demarcates the forebrain anlage, indicating the onset of forebrain regional specification at gastrulation.
Chick homeobox gene cDlx expression demarcates the forebrain anlage, indicating the onset of forebrain regional specification at gastrulation.
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鸡同源盒基因 cDlx 的表达界定了前脑原基，表明原肠胚时前脑区域规范的开始。
DOI：
10.1159/000017441
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发表时间：
2000
影响因子：
2.9
通讯作者：
Siddiqui,MA
中科院分区：
文献类型：
--
作者：
Borghjid,S;Siddiqui,MA
在这里，我们描述了含有鸡同源异型盒的基因 cDlx 的分离和表征，该基因与其他脊椎动物远端较少基因的同源域具有超过 85% 的同源性。 Northern印迹分析和原位杂交研究表明cDlx表达受发育调节且具有组织特异性。特别地，发育表达模式的特征在于cDlx转录物早期出现在原肠胚的预期前脑区域中。在神经形成过程中，cDlxi 在神经板的假定腹侧前脑区域的空间受限区域中一致表达，该神经板将产生下丘脑和腺垂体。我们的数据支持这样的观点，即Dlx基因家族的成员是前脑模式形成中同源盒基因代码的一部分，并表明前脑的区域规范发生的阶段比以前认为的要早得多。因此，同源框基因cDlx可能早在原肠胚形成时就在定义前脑区域特征中发挥了作用。
Here we describe the isolation and characterization of a chick homeobox-containing gene,cDlx, which shows greater than 85% homology to the homeodomain of other vertebrateDistal-lessgenes. Northern blot analysis and in situ hybridization studies reveal thatcDlxexpression is developmentally regulated and is tissue specific. In particular, the developmental expression pattern is characterized by an early appearance ofcDlxtranscript in the prospective forebrain region of gastrulating embryos. During neurulation,cDlxis consistently expressed in a spatially restricted domain in the presumptive ventral forebrain region of the neural plate that will give rise to the hypothalamus and the adenohypophysis. Our data support the notion that members of theDlxgene family are part of a homeobox gene code in forebrain pattern formation and suggest that regional specification of the forebrain occurs at much earlier stages than previously thought. The homeobox genecDlxmay thus play a role in defining forebrain regional identity as early as gastrulation.