Mixed Self-Assembled Monolayers in Chemical Separations
Mixed Self-Assembled Monolayers in Chemical Separations
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DOI：
10.1126/science.275.5296.44
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发表时间：
1997-01
期刊：
影响因子：
56.9
通讯作者：
M. Wirth;R. Fairbank;Hafeez O. Fatunmbi-
中科院分区：
文献类型：
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作者：
M. Wirth;R. Fairbank;Hafeez O. Fatunmbi-
许多生物分子和药物的化学分离受到它们与分离介质表面的静电相互作用的限制。十八烷基链和甲基链的混合自组装单分子膜在层析二氧化硅表面形成致密的二维交联网络，以减少表面硅烷醇的酸解离。分子模型预测，纯甲基硅氧烷单分子层在空间上是可能发生二维交联的，硅-29核磁共振测量表明，主要是甲基硅氧烷的混合单层膜以交联为主，而色谱测量证实，当单分子层主要是甲基硅氧烷时，静电相互作用减弱。对一种高电荷蛋白质细胞色素c的遗传变异的层析分离，展示了自组装单分子膜在生物分子分离中的前景。
Chemical separations of many biomolecules and pharmaceuticals are limited by their electrostatic interaction with the surfaces of the separation medium. Mixed self-assembled monolayers of octadecyl and methyl chains organize into a dense, two-dimensionally cross-linked network over the chromatographic silica surface to reduce acid dissociation of the surface silanols. Molecular models predict that two-dimensional cross-linking is sterically possible for pure methylsiloxane monolayers, silicon-29 nuclear magnetic resonance measurements show that cross-linking predominates for mixed monolayers of primarily methylsiloxane, and chromatographic measurements confirm that electrostatic interactions are reduced when the monolayer is primarily methylsiloxane. Chromatographic separation of genetic variants of a highly charged protein, cytochrome c, demonstrates the promise of self-assembled monolayers in separations of biomolecules.