Cadherin-catenin complexes during zebrafish oogenesis:: Heterotypic junctions between oocytes and follicle cells
Cadherin-catenin complexes during zebrafish oogenesis:: Heterotypic junctions between oocytes and follicle cells
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DOI：
10.1095/biolreprod61.3.692
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发表时间：
1999-09-01
影响因子：
3.6
通讯作者：
Franke, WW
中科院分区：
文献类型：
--
作者：
Cerdà, J;Reidenbach, S;Franke, WW
在脊椎动物卵子发生过程中，生殖细胞和相关的体细胞通过各种粘附的连接复合物保持连接。然而，这些细胞结构的分子组成在很大程度上是未知的。为了鉴定形成斑马鱼（Danio rerio）卵母细胞和卵泡细胞之间异型粘附连接的蛋白质，分离编码α E-连环蛋白和斑珠蛋白的cDNA。使用这些cDNA，结合先前分离的β-连环蛋白cDNA，和α-和β-连环蛋白，斑珠蛋白，N-和E-钙粘蛋白特异性抗体，我们发现在卵子发生过程中连环蛋白和斑珠蛋白基因表达的差异。这些斑块蛋白以及钙粘蛋白在卵泡中的免疫定位表明α-和β-连环蛋白以及E-钙粘蛋白样蛋白在卵母细胞皮质中富集，特别是在卵母细胞-卵泡细胞接触部位，表明这些细胞之间存在迄今未知的异型粘附连接。相比之下，斑珠蛋白和N-钙粘蛋白的定位仅限于毛囊细胞层中的细胞-细胞接触。在卵母细胞成熟过程中，α-E-和β-连环蛋白和斑珠蛋白的mRNA积累，所有三种斑块形成蛋白都储存在未受精卵中，以与钙粘蛋白复合的形式或作为游离的细胞质池。这些发现表明这些连接蛋白在早期胚胎发生过程中可能发挥的作用。
During vertebrate oogenesis, the germ cells and associated somatic cells remain connected by a variety of adhering junctional complexes. However, the molecular composition of these cellular structures is largely unknown. To identify the proteins forming the heterotypic adherens junctions between oocytes and follicle cells in the zebrafish (Danio rerio), the cDNAs encoding alpha E-catenin and plakoglobin were isolated. Using these cDNAs, in combination with the previously isolated beta-catenin cDNA, and antibodies specific for alpha- and beta-catenin, plakoglobin, and N- and E-cadherin, we found differences in catenin and plakoglobin gene expression during oogenesis. The immunolocalization of these plaque proteins, as well as of cadherins, in the ovarian follicle indicated an enrichment of alpha- and beta-catenin and of E-cadherin-like protein(s) in the oocyte cortex, notably at sites of oocyte-follicle cell contacts, suggesting the presence of hitherto unknown heterotypic adherens junctions between these cells. By contrast, plakoglobin and N-cadherin localization was restricted to cell-cell contacts in the follicle cell layer. During oocyte maturation, mRNAs for alpha E- and beta-catenin and plakoglobin accumulated, and all three plaque-forming proteins were stored in unfertilized eggs, either in complexed forms with cadherins or as free cytoplasmic pools. These findings suggest possible roles of these junctional proteins during early embryogenesis.