Lactobacillus nasalidis sp. nov., isolated from the forestomach of a captive proboscis monkey (Nasalis larvatus)
Lactobacillus nasalidis sp. nov., isolated from the forestomach of a captive proboscis monkey (Nasalis larvatus)
复制标题
鼻乳杆菌 sp.
DOI：
10.1099/ijsem.0.004787
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发表时间：
2021
影响因子：
2.8
通讯作者：
Ushida Kazunari
中科院分区：
文献类型：
--
作者：
Suzuki-Hashido Nami;Tsuchida Sayaka;Hayakawa Takashi;Sakamoto Mitsuo;Azumano Akinori;Seino Satoru;Matsuda Ikki;Ohkuma Moriya;Ushida Kazunari
从日本横滨动物园圈养的雄性长鼻猴（Nasalis larvatus）的前胃内容物中分离到3株（YZ 01 T、YZ 02和YZ 03）革兰氏染色阳性的兼性厌氧杆菌。16 S rRNA基因序列的系统发育分析表明，这些菌株属于乳杆菌属（Lactobacillus）。根据16 S rRNA基因的序列相似性，德氏乳杆菌印度亚种JCM 15610 T与YZ 01 T的系统发育关系最近。两个部分串联管家基因，RNA聚合酶α亚基（rpoA）和苯丙氨酰-tRNA合成酶α亚基（pheS）的序列分析也表明，新菌株属于属乳杆菌。结果表明，L.德氏亚种和YZ 01 T的抗性分别为85.9%和31.4%。 基于菌株YZ 01 T、YZ 02和YZ 03全基因组数据构建的系统发育树表明，这3株菌株在乳杆菌属中形成了单一的单系聚簇，表明其属于一个新种。DNA G+C含量为51.6mol%。主要脂肪酸为C16：0和C18：1ω 9 c。 因此，基于系统发育、表型和生理学证据，菌株YZ 01 T、YZ 02和YZ 03代表乳杆菌属的一个新种，其名称为鼻唾液乳杆菌（Lactobacillus nasalidissp.）nov.与模式菌株YZ 01 T（=JCM 33769 T =DSM 110539 T）。
Three strains (YZ01T, YZ02 and YZ03) of Gram-stain-positive, facultatively anaerobic rods were isolated from the forestomach contents collected from a captive male proboscis monkey (Nasalis larvatus) at Yokohama Zoo in Japan. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belonged to the genusLactobacillus. Based on the sequence similarity of the 16S rRNA gene,Lactobacillus delbrueckiisubsp.indicusJCM 15610Twas the closest phylogenetic neighbour to YZ01T. Sequence analyses of two partial concatenated housekeeping genes, the RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS) also indicated that the novel strains belonged to the genusLactobacillus. The average nucleotide identity and digital DNA–DNA hybridization (dDDH) betweenL. delbrueckiisubsp.indicusand YZ01Twere 85.9 and 31.4 %, respectively. The phylogenetic tree based on the whole genomic data of strains YZ01T, YZ02 and YZ03 suggested that these three strains formed a single monophyletic cluster in the genusLactobacillus, indicating that it belonged to a new species. The DNA G+C content of strain YZ01Twas 51.6 mol%. The major fatty acids were C16 : 0and C18 : 1ω9c. Therefore, based on phylogenetic, phenotypic and physiological evidence, strains YZ01T, YZ02 and YZ03 represent a novel species of the genusLactobacillus, for which the nameLactobacillus nasalidissp. nov. is proposed with the type strain YZ01T(=JCM 33769T=DSM 110539T).