Activin-like signaling activates Notch signaling during mesodermal induction
Activin-like signaling activates Notch signaling during mesodermal induction
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DOI：
10.1387/ijdb.041838ta
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发表时间：
2004-06-01
影响因子：
0.7
通讯作者：
Asashima, M
中科院分区：
文献类型：
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作者：
Abe, T;Furue, M;Asashima, M
激活素样信号和Notch信号在早期发育过程中都发挥着重要作用。激活素样信号参与中胚层的诱导，可以从预期的外胚层细胞(动物帽)诱导出广泛的中胚层基因和组织。另一方面，当多能前体细胞达到特定的细胞命运时，Notch信号起着重要的作用。然而，这两条信号通路之间的关系还没有被很好地理解。在这里，我们证明了激活素A诱导了Delta-1、Delta-2和Notch的表达，然后激活了动物帽子中的Notch信号。此外，在体内，异位激活素样信号诱导Delta-1和Delta-2的异位表达，而抑制激活素样信号则取消Delta-1和Delta-2的表达。此外，我们还发现，由激活素A诱导的肌源性基因MyoD可以诱导Delta-1的表达。而MyoD对Notch的表达无影响，对Delta-2的表达有抑制作用。这些结果表明激活素A通过不同的级联途径诱导Delta-1、Delta-2和Notch。我们得出的结论是，当激活素样信号诱导同种未分化细胞形成各种组织时，Notch信号被激活。
Both activin-like signaling and Notch signaling play fundamental roles during early development. Activin-like signaling is involved in mesodermal induction and can induce a broad range of mesodermal genes and tissues from prospective ectodermal cells (animal caps). On the other hand, Notch signaling plays important roles when multipotent precursor cells achieve a specific cell fate. However, the relationship between these two signal pathways is not well understood. Here, we show that activin A induces Delta-1, Delta-2 and Notch expression and then activates Notch signaling in animal caps. Also, in vivo, ectopic activin-like signaling induced the ectopic expression of Delta-1 and Delta-2, whereas inhibition of activin-like signaling abolished the expression of Delta-1 and Delta-2. Furthermore, we show that MyoD, which is myogenic gene induced by activin A, can induce Delta-1 expression. However, MyoD had no effect on Notch expression, and inhibited Delta-2 expression. These results indicated that activin A induces Delta-1, Delta-2 and Notch by different cascades. We conclude that Notch signaling is activated when activin-like signaling induces various tissues from homogenous undifferentiated cells.