Effects of selenium intake on the expression of prostaglandin-endoperoxide synthase 2 (cyclooxygenase-2) and matrix metallopeptidase-9 genes in the coronary artery disease: Selenegene study, a double-blind randomized controlled trial.
Effects of selenium intake on the expression of prostaglandin-endoperoxide synthase 2 (cyclooxygenase-2) and matrix metallopeptidase-9 genes in the coronary artery disease: Selenegene study, a double-blind randomized controlled trial.
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DOI：
10.22122/arya.v17i0.2093
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发表时间：
2021-03
影响因子：
0.6
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氧化应激被认为是健康问题的主要贡献者之一。环氧合酶-2（考克斯-2）和基质金属肽酶-9（MMP-9）是近年来发现的与心血管疾病（CVD）发生发展有关的两个重要基因。本研究的目的是评估冠状动脉疾病（CAD）患者补充硒后考克斯-2和MMP-9的表达水平。在Selenegene研究的这一子研究中，受试者被随机分组，19名受试者接受硒治疗，22名CAD患者接受安慰剂治疗。患者餐后服用200毫克硒酵母片或安慰剂片，每天一次，持续60天。在研究前后测定硒和内过氧化物合酶2（PTGS 2）（考克斯-2）和MMP-9基因产物的信使核糖核酸（mRNA）水平。在该子研究中，入组了41例伊朗CVD患者（安慰剂组：n = 22，硒干预组：n = 19）。安慰剂组空腹血糖（FBS）高于硒组（93.4 ± 12.7 vs.124.4 ± 40.6 mg/dl，P = 0.03）。硒组甘油三酯（TG）水平高于安慰剂组（123.3 ± 34.0 vs. 184.8 ± 69.4 mg/dl，P = 0.006）。数据分析表明，硒和安慰剂组MMP-9和考克斯-2基因的表达没有显著变化。本研究显示，在接受硒治疗的CAD患者中，MMP-9和考克斯-2的表达呈正相关，而安慰剂组无相关性。然而，这些发现需要进一步的细节和扩大样本量来证实。
The oxidative stress is regarded as one of the main contributors to the health problem. Cyclooxygenase-2 (COX-2) and matrix metallopeptidase-9 (MMP-9) are two of the important genes that are reported to be involved in the cardiovascular disease (CVD) development in the molecular and genetic association studies. The aim of this study was to evaluate the level of expression of COX-2 and MMP-9 after selenium supplementation in patients with coronary artery disease (CAD). In this sub-study of Selenegene study, subjects were randomly divided into groups, 19 subjects who received selenium and 22 patients with CAD who received placebo. Patients received either 200-mg selenium yeast tablets or placebo tablets after a meal, once daily for 60 days. The messenger ribonucleic acid (mRNA) levels of the selenium and prostaglandin-endoperoxide synthase 2 (PTGS2) (COX-2) and MMP-9 genes products were determined before and after the study. In this sub-study, 41 Iranian patients with CVD were enrolled (placebo group: n = 22, selenium intervention: n = 19). Fasting blood sugar (FBS) was higher among placebo group than selenium group (93.4 ± 12.7 vs. 124.4 ± 40.6 mg/dl, P = 0.03). Triglyceride (TG) level was higher among selenium group versus placebo group (123.3 ± 34.0 vs. 184.8 ± 69.4 mg/dl, P = 0.006). The data analysis demonstrated that the expression of MMP-9 and COX-2 genes did not change significantly in both selenium and placebo groups. This study showed a positive association between the expression of MMP-9 and COX-2 in the patients with CAD who received selenium but not the placebo groups. Yet, these findings need to be confirmed in further details and expanded sample size.