On the distribution and metabolism of Fusarium-toxins along the gastrointestinal tract of endotoxaemic pigs
On the distribution and metabolism of Fusarium-toxins along the gastrointestinal tract of endotoxaemic pigs
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DOI：
10.1080/1745039x.2018.1465261
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发表时间：
2018-01-01
影响因子：
2
通讯作者：
Daenicke, Sven
中科院分区：
文献类型：
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作者：
Bannert, Erik;Tesch, Tanja;Daenicke, Sven
本研究旨在探讨大肠杆菌脂多糖（LPS）对脱氧雪梨烯醇（DON）、去环氧脱氧雪梨烯醇（DOM-1）、玉米赤霉烯酮（ZEN）及其代谢产物-玉米赤霉烯醇（- zel）、-玉米赤霉烯醇（- zel）、玉米赤霉烯酮（ZAN）、-玉米赤霉烯醇（- zal）和-玉米赤霉烯醇（- zal）沿胃肠道轴在肝前或肝后给药后的潜在调节作用。饲喂自然霉菌毒素污染饲粮（4.59mg DON/kg饲料和0.22mg ZEN/kg饲料）15头犊牛，并安装颈静脉导管和门静脉导管。在第29天（第29天），在肝前或肝后分别注射LPS或对照液（LPS， 7.5 μ g/kg体重；对照，0.9% NaCl），形成3个注射组：conjux - conpo、conjux - lpspo和LPSju-CONpo。相对于注射开始195min（饲喂后210min），处死猪，收集胃和小肠（近端、中端和远端）内容物及粪便。在所有注射LPS的动物中，无论LPS进入的位置如何，都能恢复更多的干物质，这表明内毒素条件下胃排空减少，胃肠运动减弱。胃肠道（GIT）中的DON代谢未受治疗影响，包括沿GIT的DOM-1比例增加，特别是从远端小肠到粪便。描述ZEN代谢的变量表明，在lps输注组，尤其是LPSju-CONpo组，ZEN及其代谢物的胆汁释放受到刺激。综上所述，在内毒素中毒的猪体内，ZEN的GIT代谢受到显著影响，颈静脉诱导急性期反应比门静脉LPS输注更有效，这暗示了强大的肝脏首过效应。
The aim of this study was to investigate the potential modulatory effect of E. coli lipopolysaccharides (LPS) on residues of deoxynivalenol (DON), de-epoxy-deoxynivalenol (DOM-1), zearalenone (ZEN) and its metabolites -zearalenol (-ZEL), -zearalenol (-ZEL), zearalanone (ZAN), -zearalanol (-ZAL) and -zearalanol (-ZAL) after pre- or post-hepatic administration along the gastrointestinal axis. Fifteen barrows were exposed to a naturally mycotoxin contaminated diet (4.59mg DON/kg feed and 0.22mg ZEN/kg feed) and equipped with jugular (ju) and portal (po) catheters. On sampling day (day 29), the barrows were infused with LPS or a control fluid (LPS, 7.5 mu g/kg body weight; control, 0.9% NaCl) either pre- or post-hepatically, resulting in three infusion groups: CONju-CONpo, CONju-LPSpo and LPSju-CONpo. At 195min relative to infusion start (210min post-feeding), pigs were sacrificed and content of stomach and small intestine (proximal, medial and distal part) as well as faeces were collected. In all LPS-infused animals, higher amounts of dry matter were recovered irrespective of LPS entry site suggesting a reduced gastric emptying and a decreased gastrointestinal motility under endotoxaemic conditions. DON metabolism in the gastrointestinal tract (GIT) remained unaltered by treatments and included an increase in the proportion of DOM-1 along the GIT, particularly from distal small intestine to faeces. Variables describing ZEN metabolism suggest a stimulated biliary release of ZEN and its metabolites in LPS-infused groups, particularly in the LPSju-CONpo group. In conclusion, the GIT metabolism of ZEN was markedly influenced in endotoxaemic pigs whereby a jugular induction of an acute phase reaction was more effective than portal LPS infusion hinting at a strong hepatic first-pass effect.