A PCR survey of Hox genes in the myzostomid Myzostoma cirriferum
A PCR survey of Hox genes in the myzostomid Myzostoma cirriferum
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DOI：
10.1007/s00427-009-0282-z
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发表时间：
2009-04-01
影响因子：
2.4
通讯作者：
Tiedemann, Ralph
中科院分区：
文献类型：
--
作者：
Bleidorn, Christoph;Lanterbecq, Deborah;Tiedemann, Ralph
利用简并引物，我们能够鉴定出7个Myzostoma cirriferum的Hox基因。恢复的碎片属于前类（Mci_lab, Mci_pb），中心类（Mci_Dfd, Mci_Lox5, Mci_Antp, Mci_Lox4）和后类（Mci_Post2）平行群。通过系统发育分析和同源域和侧翼区域的诊断区域的存在验证了同源性分配。Lox5、Lox4和Post2的存在支持Myzostomida包含在Lophotrochozoa中。我们在Lox5的侧翼区域发现了特征残基，这在环节动物中也发现了，但在白蛉中没有。因此，myzostomids的现有Hox基因数据支持一种环节动物关系。
Using degenerate primers, we were able to identify seven Hox genes for the myzostomid Myzostoma cirriferum. The recovered fragments belong to anterior class (Mci_lab, Mci_pb), central class (Mci_Dfd, Mci_Lox5, Mci_Antp, Mci_Lox4), and posterior class (Mci_Post2) paralog groups. Orthology assignment was verified by phylogenetic analyses and presence of diagnostic regions in the homeodomain as well as flanking regions. The presence of Lox5, Lox4, and Post2 supports the inclusion of Myzostomida within Lophotrochozoa. We found signature residues within flanking regions of Lox5, which are also found in annelids, but not in Platyhelminthes. As such the available Hox genes data of myzostomids support an annelid relationship.