Effect of Various Laser-Activated Irrigation Protocols on Endodontic Sealer Penetration into the Dentinal Tubules: A Confocal Laser Scanning Microscopy Study
Effect of Various Laser-Activated Irrigation Protocols on Endodontic Sealer Penetration into the Dentinal Tubules: A Confocal Laser Scanning Microscopy Study
复制标题
各种激光激活冲洗方案对牙髓封闭剂渗透到牙本质小管的影响：共焦激光扫描显微镜研究
DOI：
10.1089/photob.2020.4924
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
He Wenxi
中科院分区：
文献类型：
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作者：
Cheng Xiaogang;Wang Xiaoli;Liu Ningning;Guan Qing;Yu Qing;He Wenxi
目的：研究不同激光激活冲洗方法对根管充填剂渗透牙本质小管的影响。背景资料：激光激活冲洗有助于去除玷污层，从而可能增加根管充填剂渗透牙本质小管的能力。方法：48颗直根管的下颌前磨牙，分为4组：C-I组，先用次氯酸钠（NaOCl）常规针冲洗，然后用乙二胺四乙酸（EDTA）; Er：YAG-I、Er，Cr：YSGG-I和Nd：YAP-I组，分别用铒激活NaOCl和EDTA冲洗;钇铝石榴石（Er：YAG）、铒、铬：钇钪镓石榴石（Er，Cr：YSGG）和钕：钇铝钙钛矿（Nd：雅普）激光器。用扫描电镜观察根管的清洁度。然后用0.1%罗丹明B标记的AH-Plus糊剂结合牙胶进行根管充填。结果：Er：YAG-I和Er，Cr：YSGG-I激光去除玷污层的效果优于Nd：YAP-I和C-I激光。CLSM结果显示，各组AH-Plus的平均渗透面积由大到小依次为：C-I < Nd：YAP-I < Er：YAG-I，Er，Cr：YSGG-I（p< 0.05）。结论：激光激活冲洗能显著增加根管充填物对牙本质小管的渗透，其中Er：YAG-I和Er，Cr：YSGG-I激光的效果最好，可作为根管治疗的常规方案。
Objective:To study the effect of various laser-activated irrigation protocols on endodontic sealer penetration into the dentinal tubules.Background data:Laser-activated irrigation is helpful for smear layer removal and thus might increase the penetration of endodontic sealer into the dentinal tubules.Methods:Forty-eight mandibular premolars with straight canals were instrumented and divided into four groups: C-I group, conventional needle irrigation with sodium hypochlorite (NaOCl) followed by ethylenediaminetetraacetic acid (EDTA); Er:YAG-I, Er,Cr:YSGG-I, and Nd:YAP-I groups, NaOCl and EDTA irrigations activated by erbium:yttrium-aluminum-garnet (Er:YAG), erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG), and neodymium:yttrium-aluminum-perovskite (Nd:YAP) lasers, respectively. The cleanness of the treated canals was evaluated by scanning electron microscopy (SEM). Then, canals were obturated with AH-Plus sealer labeled with rhodamine B (0.1%) and combined with gutta-percha. After setting, canals were sectioned at 2, 5, and 8 mm short from the root apex, and the penetration area of the AH-Plus into the dentinal tubules was observed using confocal laser scanning microscopy (CLSM).Results:SEM results indicated that the Er:YAG-I and Er,Cr:YSGG-I lasers were more effective than the Nd:YAP-I and C-I in removing the smear layer. CLSM results indicated that the average AH-Plus penetration areas among the tested groups were in ascending order: C-I < Nd:YAP-I < Er:YAG-I, Er,Cr:YSGG-I (p< 0.05).Conclusions:Laser-activated irrigations significantly increased the penetration of the endodontic sealer into the dentinal tubules, among which Er:YAG-I and Er,Cr:YSGG-I lasers were the most effective and may be considered routine protocols for endodontic treatment.