Immunohistochemical analysis of sustentacular cells in the adrenal medulla, carotid body and sympathetic ganglion of mice using an antibody against brain-type fatty acid binding protein (B-FABP).
Immunohistochemical analysis of sustentacular cells in the adrenal medulla, carotid body and sympathetic ganglion of mice using an antibody against brain-type fatty acid binding protein (B-FABP).
复制标题
使用脑型脂肪酸结合蛋白 (B-FABP) 抗体对小鼠肾上腺髓质、颈动脉体和交感神经节中的支持细胞进行免疫组织化学分析。
作者：
Pakkarato S;Chomphoo S;Kagawa Y;Owada Y;Mothong W;Iamsaard S;Sawatpanich T;Kondo H;Hipkaeo W.
很少有人注意到肾上腺支撑细胞，一些主要的组织学教科书甚至没有描述它们。本研究使用免疫光显微镜和抗脑型脂肪酸结合蛋白的抗体对支撑带细胞进行了详细的形态学描述。免疫阳性的支带细胞和突形成具有不同大小、紧密度或开放性孔的晶格。此外，免疫染色较弱且轮廓不明确的片状结构通常与突起和晶格相关。在颈动脉小体中，免疫染色的支撑细胞突与免疫染色较弱的片状结构联合形成晶格，但片状结构的晶格比肾上腺髓质更致密和坚硬，看起来像单独不同的室室。在神经节中，免疫染色的卫星细胞与紧密封闭的单个神经元一起运动。结果，免疫染色的片状结构被认为是薄而扁平的支撑细胞质膜的侧面视图，部分覆盖肾上腺髓质中的染色质细胞，并广泛分布于颈动脉体中，其方式与神经节中的卫星细胞非常相似。简而言之，由于分辨率的限制，肾上腺支撑细胞突的末端包围部分在切面上太薄/平坦，无法在免疫光显微镜下识别为不同的线条。在免疫光显微镜中，它们被认为是具有广泛扩展的实体。
Little attention has been paid to adrenal sustentacular cells, and several major histology textbooks do not even describe them. This study presents a detailed morphological description of sustentacular cells using immuno‐light microscopy and an antibody against brain‐type fatty acid‐binding protein. The immunopositive sustentacular cells and processes formed lattices with holes of various sizes and compactnesses or openness. In addition, weakly immunostained sheet‐like structures with ill‐defined contours were often associated with the processes and lattices. In the carotid body, which has traditionally been classified under the name of paraganglia in common with the adrenal medulla, immunostained sustentacular cell processes formed lattices in association with the weakly immunostained sheet‐like structures, but the lattices with sheets were more compact and rigid than the adrenal medulla, and appeared like individually distinct compartments. In the ganglion, the immunostained satellite cell processes with the sheets tightly enclosed individual neurons. As a result, the immunostained sheet‐like structures were regarded asen‐faceviews of thinly flattened sustentacular cytoplasmic envelopes partially covering the chromaffin cells in the adrenal medulla, and widely in the carotid body in a way rather similar to the satellite cells in the ganglion. In brief, the terminal enclosing portions of adrenal sustentacular cell processes, in cut‐views, were too thin/flat to be recognized as distinct lines in immuno‐light microscopy because of its resolution limit. They are recognized inen‐faceviews as entities of a substantially spacious extension in immuno‐light microscopy.