Expression patterns of engrailed and dpp in the gastropod Lymnaea stagnalis
Expression patterns of engrailed and dpp in the gastropod Lymnaea stagnalis
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DOI：
10.1007/s00427-008-0217-0
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发表时间：
2008-05-01
影响因子：
2.4
通讯作者：
Endo, Kazuyoshi
中科院分区：
文献类型：
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作者：
Iijima, Minoru;Takeuchi, Takeshi;Endo, Kazuyoshi
我们分离的全长cDNA的engliled和dpp-BMP 2/4的直向同源物从池塘蜗牛滞流和检查其表达模式在发展过程中的整体安装原位杂交。在原肠胚和担轮幼虫阶段，在推定和内陷壳腺的外周外胚层中表达，证实了其在软体动物中广泛保守的壳形成中的作用。在同一阶段，dpp-BMP 2/4在壳腺右侧外胚层和内陷的口道中表达。与腹足类普通髌骨（Patella vulgata）不同，我们的研究结果表明dpp-BMP 2/4在髌骨发育过程中可能参与了左右不对称性的形成途径，而不是在区域特化中发挥作用。stagnalis。
We isolated the full-length cDNAs of engrailed and dpp-BMP2/4 orthologues from the pond snail Lymnaea stagnalis and examined their expression patterns during development by the whole mount in situ hybridization. At the gastrula and trochophore stages, engrailed is expressed in the peripheral ectoderm of the presumptive and invaginating shell gland, corroborating its role in the shell formation that is widely conserved among molluscs. At the same stages, dpp-BMP2/4 is expressed in the right-hand side ectoderm of the shell gland and in the invaginating stomodaeum. Unlike in the gastropod Patella vulgata, our results suggested that dpp-BMP2/4 has a role in the shell formation, rather than in the regional specification and that it could be involved in the specification pathway of the left-right asymmetry of the developing shell in L. stagnalis.