Increased expression of a plant actin gene during a biotrophic interaction between round-leaved mallow, Malva pusilla, and Colletotrichum gloeosporioides f. sp malvae
Increased expression of a plant actin gene during a biotrophic interaction between round-leaved mallow, Malva pusilla, and Colletotrichum gloeosporioides f. sp malvae
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DOI：
10.1007/s004250050752
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发表时间：
1999-10-01
期刊：
影响因子：
4.3
通讯作者：
Goodwin, PH
中科院分区：
文献类型：
--
作者：
Jin, SM;Xu, RL;Goodwin, PH
半自养炭疽菌Colletotrichum gloeosporioides（Penz.）Pent. & Sacc. F. sp. malignant）和其寄主锦葵Malva pusilla（Sm.）的cDNA文库中克隆。肌动蛋白基因actA是C.胶孢菌属与其他真子囊菌相似，似乎是组成型表达。M.肌动蛋白基因。pusilla最类似于拟南芥的肌动蛋白基因之一，其独特之处在于对环境刺激如创伤有反应。actA的表达用于跟踪真菌在植物组织中的生长。低actA的表达发生，直到72-96小时后接种，然后迅速增加，相应的时间从较慢的活体营养型真菌的生长更快的坏死营养型生长的转变。相反，act 1的表达在活体营养阶段大约增加了一倍，然后在坏死营养阶段迅速下降。宿主肌动蛋白表达的增加可能是由于宿主细胞骨架重排，以响应活体营养型感染，和随后的宿主肌动蛋白表达的减少可能是由于宿主细胞破坏导致的组织浸渍在坏死。这是第一次报告的宿主肌动蛋白基因，可以增加在一个兼容的植物-病原体相互作用的表达。
Two actin genes, actA from the hemibiotrophic anthracnose fungus, Colletotrichum gloeosporioides (Penz.) Pent. & Sacc. f. sp. malvae, and act1 from its host, Malva pusilla (Sm.) were cloned from a cDNA library developed from infected host tissue. The actin gene, actA, of C. gloeosporioides f. sp. malvae, which is similar to that of other euascomycetes, appears to be expressed constitutively. The actin gene of M. pusilla is most similar to one of the actin genes of Arabidopsis thaliana that is unique in being responsive to environmental stimuli such as wounding. Expression of actA was used to follow the growth of the fungus in the plant tissue. Low actA expression occurred until 72-96 h after inoculation and then increased rapidly, corresponding with the timing of the shift from slower biotrophic fungal growth to much more rapid necrotrophic growth. In contrast, expression of act1 approximately doubled during the biotrophic phase and then rapidly declined during the necrotrophic phase. Increased host actin expression could be due to host cytoskeleton rearrangement in response to biotrophic infection, and the subsequent decrease in host actin expression could be due to host cell disruption resulting from tissue maceration during necrosis. This is the first report of a host actin gene that can increase in expression during a compatible plant-pathogen interaction.