Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punica granatum L.) juice
Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punica granatum L.) juice
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DOI：
10.1016/j.cccn.2004.04.029
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发表时间：
2004-10-01
影响因子：
5
通讯作者：
Heber, D
中科院分区：
文献类型：
--
作者：
Seeram, NP;Lee, R;Heber, D
背景资料：鞣花酸（EA）和可水解的鞣花单宁（EZN）是在水果和坚果中发现的膳食多酚，具有有效的抗氧化、抗癌和抗动脉粥样硬化的生物学特性。遗憾的是，目前还没有关于EA或Escherichia coli在人体内的生物利用度研究报告。我们进行了体内研究，其中人类受试者食用含有EA（25 mg）和甘草酸（318 mg，作为安石榴苷，主要水果鞣花单宁）的石榴汁（PJ）（180 ml）。方法：采用快速血浆提取程序，利用酸性沉淀蛋白质，然后进行HPLC-UV分析。结果：在摄入后1 h，在人血浆中检测到最大浓度（31.9 ng/ml）的EA，但在4 h后迅速消除。在1000 ~ 15.6ng/ml浓度范围内，线性关系良好（r（2）= 0.9975）。结论：据报道，由于EA对蛋白质具有强亲和力，并且在小动物中吸收较差，因此需要进一步研究以调查人血浆中游离EA的存在是否可能是由于其通过生理pH值和/或肠道微生物群落作用促进的β-内酰胺水解而释放。EA可以被认为是未来人体生物利用度研究的生物标志物，涉及从食物来源中摄入的维生素A。(C)2004 Elsevier B. V.保留所有权利。
Background: Ellagic acid (EA) and hydrolyzable ellagitannins (ETs) are dietary polyphenols found in fruits and nuts and implicated with potent antioxidant, anticancer and antiatherosclerotic biological properties. Unfortunately, there are no reports on the bioavailability studies of EA or ETs in the human body. We conducted in vivo studies whereby a human subject consumed pomegranate juice (PJ) (180 ml) containing EA (25 mg) and ETs (318 mg, as punicalagins, the major fruit ellagitannin). Methods: A rapid plasma extraction procedure utilizing acidic precipitation of proteins, followed by HPLC-UV analyses, was employed. Results: EA was detected in human plasma at a maximum concentration (31.9 ng/ml) after 1 h post-ingestion but was rapidly eliminated by 4 h. The calibration curve for quantification of EA was linear (r(2) = 0.9975) over the concentration range from 1000 to 15.6 ng/ml. Conclusions: Since EA has reportedly strong affinity for proteins and poor absorption in small animals, further studies to investigate whether the presence of free EA in human plasma may be due to its release from the hydrolysis of ETs, facilitated by physiological pH and/or gut microflora action, is warranted. EA can be considered as a biomarker for future human bioavailability studies involving consumption of ETs from food sources. (C) 2004 Elsevier B.V. All rights reserved.