Measuring Inaccessible Chromatin Genome-Wide Using Protect-seq.
Measuring Inaccessible Chromatin Genome-Wide Using Protect-seq.
复制标题
使用 Protect-seq 在全基因组范围内测量不可接近的染色质。
DOI：
10.1007/978-1-0716-2899-7_4
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Dekker,Job
中科院分区：
文献类型：
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作者：
Spracklin,George;Yang,Liyan;Pradhan,Sriharsa;Dekker,Job
染色质可及性已经成为识别和理解基因组中调控元件的一个非常强大的指标。许多重要的调控元件，如增强子和转录起始位点，其特征在于“开放”或无核小体的区域。要了解那些不被认为是开放染色质的基因组区域就更加困难了。Protect-seq是一种基因组学技术，旨在识别与核周边相关的不可接近的染色质。这些区域富含与转录抑制相关的组蛋白修饰，并与通过测量异染色质和外周定位的其他技术鉴定的基因座相关。在这里，我们讨论执行Protect-seq的协议和最佳实践。
Chromatin accessibility has been an immensely powerful metric for identifying and understanding regulatory elements in the genome. Many important regulatory elements, such as enhancers and transcriptional start sites, are characterized by “open” or nucleosome-free regions. Understanding the areas of the genome that are not considered open chromatin has been more difficult. Protect-seq is a genomics technique that aims to identify inaccessible chromatin associated with the nuclear periphery. These regions are enriched for histone modifications associated with transcriptional repression and correlate with loci identified by other techniques measuring heterochromatin and peripheral localization. Here, we discuss the protocol and best practices to perform Protect-seq.