The Use of Bone Marrow Stromal Cells (Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells) for Alveolar Bone Tissue Engineering: Basic Science to Clinical Translation
The Use of Bone Marrow Stromal Cells (Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells) for Alveolar Bone Tissue Engineering: Basic Science to Clinical Translation
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DOI：
10.1089/ten.teb.2013.0578
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发表时间：
2014-06-01
影响因子：
6.4
通讯作者：
Imai, Kohzoh
中科院分区：
文献类型：
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作者：
Kagami, Hideaki;Agata, Hideki;Imai, Kohzoh
骨组织工程是再生医学的一个很有前途的领域，在该领域中，培养的细胞、支架和成骨诱导信号被用于骨再生。人骨髓基质细胞是骨组织工程中最常用的细胞来源。虽然已知细胞培养和诱导方案显著影响BMSCs的体内成骨能力，但对临床结果的责任因素知之甚少。最近使用人BMSCs的研究结果表明，传代次数和成骨诱导时间等因素显著影响异位骨形成，尽管这些差异几乎不影响碱性磷酸酶活性或成骨标志物的基因表达。碱性成纤维细胞生长因子的应用有助于维持BMSCs体内成骨能力。重要的是，这些因素的反应性应在临床情况下进行测试，以进一步提高骨组织工程。本文就骨组织工程的临床应用及其可能机制作一综述。
Bone tissue engineering is a promising field of regenerative medicine in which cultured cells, scaffolds, and osteogenic inductive signals are used to regenerate bone. Human bone marrow stromal cells (BMSCs) are the most commonly used cell source for bone tissue engineering. Although it is known that cell culture and induction protocols significantly affect the in vivo bone forming ability of BMSCs, the responsible factors of clinical outcome are poorly understood. The results from recent studies using human BMSCs have shown that factors such as passage number and length of osteogenic induction significantly affect ectopic bone formation, although such differences hardly affected the alkaline phosphatase activity or gene expression of osteogenic markers. Application of basic fibroblast growth factor helped to maintain the in vivo osteogenic ability of BMSCs. Importantly, responsiveness of those factors should be tested under clinical circumstances to improve the bone tissue engineering further. In this review, clinical application of bone tissue engineering was reviewed with putative underlying mechanisms.