Resveratrol alleviates oxidative stress caused by Streptococcus uberis infection via activating the Nrf2 signaling pathway
Resveratrol alleviates oxidative stress caused by Streptococcus uberis infection via activating the Nrf2 signaling pathway
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白藜芦醇通过激活Nrf2信号通路减轻乳房链球菌感染引起的氧化应激
DOI：
10.1016/j.intimp.2020.107076
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发表时间：
2020
影响因子：
5.6
通讯作者：
Miao jinfeng
中科院分区：
文献类型：
--
作者：
Zhou Yilin;Lan Riguo;Xu yuanyuan;Zhou yuanyuan;Lin Xinguang;Miao jinfeng
由于其抗氧化特性，白藜芦醇可以减轻链球菌（S。泌尿系统）感染。然而，潜在的分子机制仍然未知。本研究以白藜芦醇为实验材料，以C57 BL/6小鼠和小鼠乳腺上皮细胞株（EpH 4-Ev）为实验材料，探讨白藜芦醇对宿主氧化损伤的调控分子机制。结果表明，灌胃白藜芦醇可通过激活Nrf 2信号通路减轻链球菌感染引起的乳腺组织炎症反应和氧化损伤。为了进一步了解其分子机制，在乳腺上皮细胞中使用Nrf 2抑制剂（ML 385）和靶向p62的siRNA。结果表明，白藜芦醇通过激活p62介导Keap 1降解，诱导Nrf 2及其下游抗氧化信号通路的表达，改善S.总的来说，这些结果表明白藜芦醇可以作为p62-Keap 1/Nrf 2信号通路的激活剂来改善由S.在乳腺中以及在EpH 4-Ev细胞中表达。因此，白藜芦醇可能对防治S.缓解氧化应激诱导奶牛乳房炎
Due to its antioxidant properties, resveratrol may relieve the cellular oxidative injury induced by Streptococcus uberis (S. uberis) infection. However, the underlying molecular mechanisms remain unknown. Herein, we used S.uberis to challenge C57BL/6 mice or a mouse mammary epithelial cell line (EpH4-Ev), and the regulatory molecular mechanism of resveratrol on hosts’ oxidative injury were investigated. The results showed that gavage of resveratrol alleviate the inflammatory responses and oxidative injury of mammary gland tissues induced by S.uberis infection via activating Nrf2 signaling pathways. To further understand the molecular mechanism, inhibitor of Nrf2 (ML385) and siRNA targeting p62 were used in mammary epithelial cells. The findings indicated that resveratrol mediates Keap1 degradation by activating p62, induces the expression of Nrf2 and its down stream antioxidant signaling pathways, and ameliorates oxidative damage during S. uberis infection.Collectively, these outcomes suggested that resveratrol can function as an activator of the p62–Keap1/Nrf2 signaling pathway to improve oxidative injury caused by S. uberis in mammary glands as well as in EpH4-Ev cells. Therefore, resveratrol may be useful to prevent and control S. uberis-induced bovine mastitis by relieving oxidative stress