High levels of viral replication during primary simian immunodeficiency virus SIVagm infection are rapidly and strongly controlled in African green monkeys
High levels of viral replication during primary simian immunodeficiency virus SIVagm infection are rapidly and strongly controlled in African green monkeys
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DOI：
10.1128/jvi.74.16.7538-7547.2000
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发表时间：
2000-08-01
影响因子：
5.4
通讯作者：
Müller-Trutwin, MC
中科院分区：
文献类型：
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作者：
Diop, OM;Gueye, P;Müller-Trutwin, MC
与致病性人类免疫缺陷病毒和猿猴免疫缺陷病毒（SIV）感染相反，非洲绿色猴（AGNl）中的慢性SIVagm感染的特征在于持续低的外周和组织病毒载量，这与在这些动物中观察到的疾病缺乏相关。我们在此报道了AGM中急性SIVagm感染的动力学数据，其表现出与在致病性SIVmac感染的前2周期间在外周血中观察到的病毒复制模式的显著相似性。在AGM中，快速病毒复制导致第7 - 10天达到病毒高峰，特征为高水平抗原血症（1.2 - 5 ng p27/ml血浆）、外周DNA病毒载量（10（4）-10（5）DNA拷贝/10（6）外周血单核细胞[PBMC]）和血浆RNA病毒载量（2 x 10（6）至2 x 10（8）RNA拷贝/ml），淋巴结（LN）RNA和DNA病毒载量模式与血液中的相似，在第7天和第14天之间观察到峰值，LN中的这些值（范围为3 x 10（5）至3 x 10（6）RNA拷贝/10（6）LN细胞[LNC]和10（3）至10（4）DNA拷贝/10（6）LNC）在任何时间点均不高于血液中观察到的值。在LN和血液中，在病毒复制高峰后，在所有感染动物中观察到快速和显著的下降。感染后3 - 1周内，不再检测到抗原血症，外周病毒载量降低至与感染慢性期特征相似的值（10（2）-10（3）DNA拷贝/10（6）PBMC和2 x 10（3）-2 x 10（5）RNA拷贝/ml血浆）。在接种后第28天，LN中的病毒载量下降至每10（6）LNC 5 × 10（1）至10（3）DNA拷贝和10（4）至3 × 10（5）RNA拷贝。并持续下降，直到感染后第84天，（
In contrast to pathogenic human immunodeficiency virus and simian immunodeficiency virus (SIV) infections, chronic SIVagm infections in African green monkeys (AGNls) are characterized by persistently low peripheral and tissue viral loads that correlate with the Lack of disease observed in these animals. We report here data on the dynamics of acute SIVagm infection in AGMs that exhibit remarkable similarities with viral replication patterns observed in peripheral blood during the first 2 weeks of pathogenic SIVmac infections, Plasma viremia was evident at day 3 postinfection (p,i.) in AGMs, and rapid viral replication led by days 7 to 10 to peak viremias characterized by high levels of antigenemia (1.2 to 5 ng of p27/ml of plasma), peripheral DNA viral load (10(4) to 10(5) DNA copies/10(6) peripheral blood mononuclear cells [PBMC]), and plasma RNA viral load (2 x 10(6) to 2 x 10(8) RNA copies/ml), The lymph node (LN) RNA and DNA viral load patterns were similar to those in blood, with peaks observed between day 7 and day 14, These values in LNs (ranging from 3 x 10(5) to 3 x 10(6) RNA copies/10(6) LN cell [LNC] and 10(3) to 10(4) DNA copies/10(6) LNC) were at no time point higher than those observed in the blood. Both in LNs and in blood, rapid and significant decreases were observed in all infected animals after this peak of viral replication. Within 3 to I weeks p.i,, antigenemia was no longer detectable and peripheral viral loads decreased to values similar to those characteristic of the chronic phase of infection (10(2) to 10(3) DNA copies/10(6) PBMC and 2 x 10(3) to 2 x 10(5) RNA copies/ml of plasma). In LNs, viral loads declined to 5 x 10(1) to 10(3) DNA copies and 10(4) to 3 X 10(5) RNA copies per 10(6) LNC at day 28 p.i. and continued to decrease until day 84 p.i, (