Fetomodulin: marker surface protein of fetal development which is modulatable by cyclic AMP.
Fetomodulin: marker surface protein of fetal development which is modulatable by cyclic AMP.
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Fetomodulin：胎儿发育的标记表面蛋白，可由环 AMP 调节。
DOI：
10.1016/0012-1606(87)90312-5
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发表时间：
1987
影响因子：
2.7
通讯作者：
Moore,EE
中科院分区：
文献类型：
--
作者：
Imada,M;Imada,S;Iwasaki,H;Kume,A;Yamaguchi,H;Moore,EE
用抗表观分子量为133，000 Da的糖蛋白的单克隆抗体，在小鼠体内和体外系统中鉴定出一种新的胎儿发育的细胞表面标志物，并将小鼠骨髓瘤细胞NS-1与用小鼠3 T3成纤维细胞免疫的大鼠脾细胞融合，获得两个独立的杂交瘤克隆。免疫沉淀物的分子大小和胰蛋白酶肽的分析表明，成纤维细胞和假定的壁内胚层细胞，这是由诱导分化的F9胚胎癌细胞与维甲酸和环AMP，表达明显相同的蛋白。未分化的F9细胞和单独用视黄酸或环AMP处理的F9细胞几乎没有或没有免疫沉淀蛋白。类似地，在体胚胎的壁内胚层检测到这种蛋白，但内脏内胚层和胚胎外胚层没有。这种表面蛋白的量在成纤维细胞和分化的F9细胞中通过升高细胞内环AMP浓度而增加。这些结果是一致的假设，即这种表面蛋白质通过环AMP的定量调节在胎儿发育中发挥作用。
A novel cell surface marker of fetal development was identified in bothin vivoandin vitrosystems of the mouse using monoclonal antibodies against a glycoprotein of an apparent size of 133,000 Da. Two independent clones of hybridomas were isolated by fusing murine myeloma cells, NS-1, with spleen cells of a rat which was immunized with murine 3T3 fibroblast. The analysis of molecular size and tryptic peptides of the immunoprecipitate indicated that fibroblast and putative parietal endoderm cells, which were derived by induced differentiation of F9 embryonal carcinoma cells with retinoic acid and cyclic AMP, expressed apparently the same protein. Undifferentiated F9 cells and F9 cells which were treated with retinoic acid or cyclic AMP alone had little or no immunoprecipitable proteins. Analogously, parietal endoderm ofin vivoembryos tested positive for this protein but visceral endoderm and embryonic ectoderm did not. The amount of this surface protein was increased in fibroblast and differentiated F9 cells by elevation of intracellular cyclic AMP concentrations. These results are consonant with a hypothesis that this surface protein plays a role in fetal development via a quantitative modulation by cyclic AMP.