Green synthesis of ZnO nanoparticles using Tecoma castanifolia leaf extract: Characterization and evaluation of its antioxidant, bactericidal and anticancer activities
Green synthesis of ZnO nanoparticles using Tecoma castanifolia leaf extract: Characterization and evaluation of its antioxidant, bactericidal and anticancer activities
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DOI：
10.1016/j.microc.2018.11.022
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发表时间：
2019-03-01
影响因子：
4.8
通讯作者：
Muthukumaran, Chandrasekaran
中科院分区：
文献类型：
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作者：
Sharmila, Govindasamy;Thirumarimurugan, Marimuthu;Muthukumaran, Chandrasekaran
报道了一种高效、简便、绿色的以柚木叶提取物为原料合成氧化锌纳米颗粒的方法。采用紫外-可见光谱、透射电镜、能谱仪、X射线衍射和红外光谱对纳米ZnO进行了表征。T.通过GC-MS分析栗叶提取物。UV-Vis吸收在370-400 nm处显示SPR带，这证实了ZnO纳米颗粒的形成。TEM分析显示为球形，尺寸为70-75 nm，XRD结果显示为六方纤锌矿结构。FTIR光谱证实了O-H、C-H、酰胺基、酰胺基、C-O键和金属氧基团的存在。研究了木霉甲醇提取物中的植物化学活性成分。经GC-MS鉴定，该提取物对革兰氏阳性菌和革兰氏阴性菌均有较好的抑菌活性。抗氧化活性的结果表明，ZnO纳米粒子的浓度增加增加的自由基清除活性。抗肿瘤活性的IC_（50）值为65 μ g/mL，表明ZnO纳米颗粒对A549细胞的增殖具有较好的细胞毒作用。本研究揭示了存在于绿色合成的ZnO纳米颗粒中的具有生物活性的化合物为其在生物医学和纳米药物递送系统中的有效应用铺平了道路。
An efficient, facile and green synthesis of zinc oxide nanoparticles (ZnO NPs) using Tecoma castanifolia leaf extract was reported. ZnO NPs was characterized by UV-Vis spectroscopy, TEM, EDX, XRD and FTIR. Phytochemical constituents of T. castanifolia leaf extract were analyzed by GC-MS. UV-Vis absorption showed SPR band at 370-400 nm which confirms the formation of ZnO NPs. TEM analysis exhibits spherical shape with size 70-75 nm and XRD results revealed the hexagonal phase of wurtzite structure. FTIR spectra confirmed the presence of O-H, C-H, amide-I, II groups, C-O bond and metal-oxygen groups. The presence of bioactive phytochemical constituents in the methanolic extracts of T. castanifolia was identified by GC-MS. An excellent antibacterial activity was observed for both Gram positive and Gram negative bacteria. Results of antioxidant activity showed that increase in concentration of ZnO nanoparticles increases the radical scavenging activity. Anticancer activity with IC50 value as 65 mu g/mL which conferred better cytotoxic effects of ZnO NPs on proliferation of A549 cell line. The present study revealed that the pharmacologically active compounds present in the green synthesized ZnO nanoparticles pave the way to lead its effective application in biomedical and nano drug delivery systems.