Effects of cryostorage on human sperm chromatin integrity
Effects of cryostorage on human sperm chromatin integrity
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DOI：
10.1017/s0967199412000032
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发表时间：
2013-11-01
期刊：
影响因子：
1.7
通讯作者：
Liguori, Francesca
中科院分区：
文献类型：
--
作者：
Fortunato, Adriana;Leo, Rita;Liguori, Francesca
精子染色质结构的完整性已被证明对人类的生育能力非常重要。在这项研究中，我们调查了精子冷冻保存是否对核DNA三级结构（即冷凝）的影响，通过苯胺蓝染色测量，103名男性患者因生育能力低下而就诊。精子DNA损伤在表现为少精症和严重形态异常的患者中明显高于原生精子群体。此外，我们观察到染色质去浓缩与低温储存技术和储存时间有关。在冷冻保存后立即和从冷冻保存90天后开始，这种去致密化的增加非常显著（P < 0.01）。本文讨论了精子染色质低温损伤的可能机制，以及将检测精子核结构改变的新方法纳入常规精子图的必要性。
The integrity of sperm chromatin structure has proven to be of great importance for human fertility. In this study, we investigated whether sperm cryopreservation has an effect on nuclear DNA tertiary structure, (i.e. condensation), measured by aniline blue staining, in 103 male patients who required consultation for hypo-fertility. Sperm DNA damage was significantly higher in patients showing oligospermia and severe morphological abnormalities than in native sperm populations. Furthermore we observed that chromatin decondensation was related to the cryostorage technique and to the duration of storage. This increase in decondensation was highly significant (P < 0.01) immediately after cryopreservation and from 90 days of cryostorage onwards. The possible mechanisms involved in sperm chromatin cryoinjury and the need to incorporate new methods for testing sperm nuclear structure alteration into the routine spermiogram are discussed.