Dynamic equilibrium between the two conformational states of spin-labeled tropomyosin.
Dynamic equilibrium between the two conformational states of spin-labeled tropomyosin.
复制标题
自旋标记原肌球蛋白的两种构象状态之间的动态平衡。
DOI：
10.1021/bi00307a011
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Lehrer,SS
中科院分区：
文献类型：
--
作者：
Graceffa,P;Lehrer,SS
Philip Graceffa* and Sherwin S. Lehrer abstract: Tropomyosin was labeled with a maleimide nitr-oxide spin-label attached to cysteine-190 via a succinimido ring which was subsequently opened by incubation at alkalinepH. Electron spin resonance(ESR) spectra showed a temperature-dependent equilibrium, below the main unfolding tran-sition of tropomyosin, between labels which were restricted in their motion (strongly immobilized), predominating at low temperatures, and those which were highly mobile (weakly immobilized), predominating at higher temperatures. These label states were associated with two protein states from a comparison of the ESR spectral changes with the thermal unfolding profile of tropomyosin. The strongly immobilized labels were associated with the completely folded molecule and the weakly immobilized labels with a partially unfolded (in the cysteine-190 region) state which is an intermediate in the
