Studying plant autophagy: challenges and recommended methodologies
Studying plant autophagy: challenges and recommended methodologies
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DOI：
10.1007/s44307-023-00002-8
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发表时间：
2023-10
期刊：
影响因子：
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通讯作者：
Hua Qi;Yao Wang;Yan Bao;D. Bassham;Liang Chen;Qin-Fang Chen;Suiwen Hou;Inhwan Hwang;
中科院分区：
文献类型：
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作者：
Hua Qi;Yao Wang;Yan Bao;D. Bassham;Liang Chen;Qin-Fang Chen;Suiwen Hou;Inhwan Hwang;
在植物中，自噬是一个保守的过程，通过该过程，细胞内物质，包括受损的蛋白质、聚集体和整个细胞器，被运输到液泡中降解，从而维持细胞内稳态。在过去的几十年里，人们对自噬机制的核心组成部分及其在植物生长发育以及对生物和非生物胁迫的响应中的生理作用进行了广泛的研究。此外，还建立了多种监测植物自噬活性的方法，为植物自噬的研究提供了极大的便利。然而，其中一些方法容易被滥用或误解，有时会让人对有关植物自噬的结论的可靠性产生怀疑。本文总结了目前在生理、微观和生化水平上广泛用于监测植物自噬的方法，并讨论了它们的优点和局限性，以期为研究这一重要过程提供指导。
In plants, autophagy is a conserved process by which intracellular materials, including damaged proteins, aggregates, and entire organelles, are trafficked to the vacuole for degradation, thus maintaining cellular homeostasis. The past few decades have seen extensive research into the core components of the central autophagy machinery and their physiological roles in plant growth and development as well as responses to biotic and abiotic stresses. Moreover, several methods have been established for monitoring autophagic activities in plants, and these have greatly facilitated plant autophagy research. However, some of the methodologies are prone to misuse or misinterpretation, sometimes casting doubt on the reliability of the conclusions being drawn about plant autophagy. Here, we summarize the methods that are widely used for monitoring plant autophagy at the physiological, microscopic, and biochemical levels, including discussions of their advantages and limitations, to provide a guide for studying this important process.