Comparison of MultiMap and TSP/CONCORDE for constructing radiation hybrid maps
Comparison of MultiMap and TSP/CONCORDE for constructing radiation hybrid maps
复制标题
DOI：
10.1093/jhered/esg012
复制
发表时间：
2003-01-01
影响因子：
3.1
通讯作者：
Galibert, F
中科院分区：
文献类型：
--
作者：
Hitte, C;Lorentzen, TD;Galibert, F
辐射杂交（RH）图谱的构建允许研究人员在给定的基因组图谱上定位I型和II型标记。该过程由两个步骤组成。第一个包括确定RH面板的不同细胞系内的一组标记物的模式分布。这主要是通过聚合酶链反应（PCR）扩增和凝胶电泳完成的，并产生一系列数字，表明每个标记在每个细胞中的存在或不存在。第二步包括使用各种算法对这些数字进行比较，以对标记进行分组，然后对标记进行排序。由于不同的算法可能提供（稍微）不同的顺序，我们比较了MultiMap和TSP/CONCORDE软件包的优点，使用目前正在分析的信息数据集构建犬基因组RH图。
Radiation hybrid (RH) map construction allows investigators to locate both type I and type II markers on a given genome map. The process is composed of two steps. The first consists of determining the pattern distribution of a set of markers within the different cell lines of an RH panel. This is mainly done by polymerase chain reaction (PCR) amplification and gel electrophoresis, and results in a series of numbers indicating the presence or the absence of each marker in each cell fine. The second step consists of a comparison of these numbers, using various algorithms, to group and then order markers. Because different algorithms may provide (slightly) different orders, we have compared the merits of the MultiMap and TSP/CONCORDE packages using a data set of information currently under analysis for construction of the canine genome RH map.