The proliferative actions of insulin, insulin-like growth factor-I, epidermal growth factor, butyrate and propionate on ruminal epithelial cells in vitro
The proliferative actions of insulin, insulin-like growth factor-I, epidermal growth factor, butyrate and propionate on ruminal epithelial cells in vitro
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DOI：
10.1016/s0921-4488(98)00188-6
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发表时间：
1999-05-01
影响因子：
1.8
通讯作者：
Baldwin, RL
中科院分区：
文献类型：
--
作者：
Baldwin, RL
瘤胃上皮的大小和表面积增加，以响应增加的采食量，可能是由于增加上皮细胞增殖。体外培养瘤胃细胞，研究不同生长因子对瘤胃细胞增殖的影响。评价的生长因子包括胰岛素、胰岛素样生长因子-I和表皮生长因子。还评价了VFA丙酸盐和丁酸盐。通过连续胰蛋白酶消化从5只羔羊中分离瘤胃上皮细胞，并通过在无菌缓冲盐溶液中连续洗涤制备用于无菌培养。通过[3-（4，5-二甲基噻唑-2-基）-2，5-二苯基-四唑溴化物]（MTT）测定来评估细胞增殖。将细胞生长刺激至在补充有5%FCS的DMEM中培养的细胞生长的75%、96%和97%（分别用于Ins、IGF-I和EGE）。丙酸盐和丁酸盐的添加导致细胞生长总体下降至低于对照（分别为61%和49%）。当丁酸盐（1 mmol）存在于培养基Ins中时，IGF-I和EGF克服了丁酸盐的抑制，但没有达到在未受抑制的培养物中观察到的生长（分别为67，57和60%）。虽然丁酸盐可能是体内瘤胃细胞生长的介质，但生长因子如胰岛素、IGF-I和EGF是体外生长的更有效诱导剂。(C)1999 Elsevier Science B. V.保留所有权利。
The rumen epithelium increases in size and surface area in response to increased feed intake, presumably due to increased epithelial cell proliferation. Isolated ruminal cells were cultured in vitro to determine the effect of various growth factors on ruminal cell proliferation. The growth factors evaluated included insulin, insulin-like growth factor-I and epidermal growth factor. VFA propionate and butyrate were also evaluated. Ruminal epithelial cells were isolated from five lambs via serial tryptic digestion and prepared for sterile culture by serial washes in sterile buffered salts solution. Cell proliferation was assessed by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetra-zoliumbromide] (MTT) assay. Cell growth was stimulated to 75, 96 and 97% of the growth of cells cultured in DMEM supplemented with 5% FCS (for Ins, IGF-I and EGE respectively). Propionate and butyrate addition resulted in an overall decline in cell growth to below controls (61 and 49%, respectively). When butyrate (1 mmol) was present in the media Ins, IGF-I and EGF overcame the butyrate inhibition, but did not attain the growth observed in uninhibited cultures (67, 57 and 60%, respectively). Although butyrate has been implicated as a possible mediator of ruminal cell growth in vivo, growth factors such as insulin, IGF-I and EGF are more effective inducers of growth in vitro. (C) 1999 Elsevier Science B.V. All rights reserved.