DEVELOPMENT OF SENSORY INNERVATION IN CHICK SKIN - COMPARISON OF NERVE-FIBER AND CHONDROITIN SULFATE DISTRIBUTIONS IN-VIVO AND IN-VITRO
DEVELOPMENT OF SENSORY INNERVATION IN CHICK SKIN - COMPARISON OF NERVE-FIBER AND CHONDROITIN SULFATE DISTRIBUTIONS IN-VIVO AND IN-VITRO
复制标题
DOI：
10.1007/bf00300225
复制
发表时间：
1994-09-01
影响因子：
3.6
通讯作者：
SAXOD, R
中科院分区：
文献类型：
--
作者：
HEMMING, FJ;PAYS, L;SAXOD, R
在鸟类皮肤中，神经纤维在真皮中发育，但不进入表皮。在7日龄鸡胚背根神经节与表皮共培养时，神经突也避开表皮。以前的研究表明，硫酸软骨素蛋白聚糖可能参与其中。因此，硫酸软骨素已可视化免疫细胞化学，使用单克隆抗体CS-56，在体内和体外使用光学和电子显微镜。它的分布进行了比较，其他2个硫酸软骨素表位和生长的神经纤维。在培养的表皮从7日龄的鸡胚，免疫反应被发现均匀的表皮细胞周围，而在7.5天的真皮中的分布是不均匀的，特别是显着的羽芽。在体内，在表皮、基底层、成纤维细胞表面和沿着胶原原纤维中检测到硫酸软骨素免疫反应性。这种定位与皮神经的分布是互补的。基片中的硫酸软骨素可以阻止表皮的神经支配，真皮的不均匀性可以部分解释羽毛基部周围的神经纤维。因此，硫酸软骨素可能是重要的神经指导发育中的鸡皮肤。
In bird skin, nerve fibres develop in the dermis but do not enter the epidermis. In co-cultures of 7-day-old chick embryo dorsal root ganglia and epidermis, the neurites also avoid the epidermis. Previous studies have shown that chondroitin sulphate proteoglycans may be involved. Chondroitin sulphate has therefore been visualized by immunocytochemistry, using the monoclonal antibody CS-56, both in vivo and in vitro using light and electron microscopy. Its distribution was compared to those of 2 other chondroitin sulphate epitopes and to that of the growing nerve fibres. In cultures of epidermis from 7-day-old embryonic chicks, immunoreactivity is found uniformly around the epidermal cells while at 7.5 days the distribution in dermis is heterogeneous, and particularly marked in feather buds. In vivo, chondroitin sulphate immunoreactivity is detected in the epidermis, on the basal lamina, on the surfaces of fibroblasts and along collagen fibrils. This localization is complementary to the distribution of cutaneous nerves. Chondroitin sulphate in the basal lamina could prevent innervation of the epidermis and the dermal heterogeneities could partly explain the nerve fibres surrounding the base of the feathers. Chondroitin sulphate could therefore be important for neural guidance in developing chick skin.