TCP transcription factors are critical for the coordinated regulation of ISOCHORISMATE SYNTHASE 1 expression in Arabidopsis thaliana
TCP transcription factors are critical for the coordinated regulation of ISOCHORISMATE SYNTHASE 1 expression in Arabidopsis thaliana
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TCP 转录因子对于拟南芥异分支合成酶 1 表达的协调调节至关重要
DOI：
10.1111/tpj.12803
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发表时间：
2015-04-01
期刊：
影响因子：
7.2
通讯作者：
Kuai, Benke
中科院分区：
文献类型：
--
作者：
Wang, Xiaoyan;Gao, Jiong;Kuai, Benke
水杨酸（SA）在植物发育和应激反应的各个方面发挥着重要作用。为了阐明SA合成和信号转导的复杂调控机制，我们使用酵母单杂交系统来筛选异分支酸合酶1（ICS1）的调节因子，ICS1是编码拟南芥SA生物合成关键酶的基因。 TCP 家族转录因子 AtTCP8 最初被确定为 ICS1 的候选调节因子。 ICS1 启动子中存在典型的 TCP 结合位点支持 TCP 蛋白对 ICS1 的调节。 TCP8 与该位点的结合通过体外和体内测定得到证实。 TCP8及其相应基因TCP9在病原体攻击下的表达模式与ICS1很大程度上重叠。在 tcp8 tcp9 双突变体中观察到免疫反应期间 ICS1 表达显着降低。我们还检测到 TCP8 与 SAR 缺陷 1 (SARD1)、WRKY 家族转录因子 28 (WRKY28)、NAC (NAM/ATAF1,ATAF2/CUC2) 家族转录因子 019 (NAC019) 之间以及 TCP8、TCP9 和 TCP20 之间的强相互作用，表明 ICS1 表达背后存在复杂的协调调节机制。我们的结果共同证明 TCP 蛋白参与 ICS1 表达的精心调控，其中 TCP8 和 TCP9 被验证为主要代表。
Salicylic acid (SA) plays an important role in various aspects of plant development and responses to stresses. To elucidate the sophisticated regulatory mechanism of SA synthesis and signaling, we used a yeast one-hybrid system to screen for regulators of ISOCHORISMATE SYNTHASE1 (ICS1), a gene encoding the key enzyme in SA biosynthesis in Arabidopsis thaliana. A TCP family transcription factor AtTCP8 was initially identified as a candidate regulator of ICS1. The regulation of ICS1 by TCP proteins is supported by the presence of a typical TCP binding site in the ICS1 promoter. The binding of TCP8 to this site was confirmed by invitro and invivo assays. Expression patterns of TCP8 and its corresponding gene TCP9 largely overlapped with ICS1 under pathogen attack. A significant reduction in the expression of ICS1 during immune responses was observed in the tcp8 tcp9 double mutant. We also detected strong interactions between TCP8 and SAR deficient 1 (SARD1), WRKY family transcription factor 28 (WRKY28), NAC (NAM/ATAF1,ATAF2/CUC2) family transcription factor 019 (NAC019), as well as among TCP8, TCP9 and TCP20, suggesting a complex coordinated regulatory mechanism underlying ICS1 expression. Our results collectively demonstrate that TCP proteins are involved in the orchestrated regulation of ICS1 expression, with TCP8 and TCP9 being verified as major representatives.