Vicianin, prunasin, and beta-cyanoalanine in common vetch seed as sources of urinary thiocyanate in the rat.
Vicianin, prunasin, and beta-cyanoalanine in common vetch seed as sources of urinary thiocyanate in the rat.
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野豌豆种子中的豌豆素、普鲁那辛和β-氰基丙氨酸是大鼠尿硫氰酸盐的来源。
DOI：
10.1021/jf010343w
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发表时间：
2001
影响因子：
6.1
通讯作者：
Tatake,JG
中科院分区：
文献类型：
--
作者：
Ressler,C;Tatake,JG
当幼鼠被喂食含有野豌豆种子的食物1个月时，它们在尿液中排出的硫氰酸盐比它们摄入的硫氰酸盐多约7倍。 Viciianin、pranasin 和 β-cyanoalanine 被确定为排泄硫氰酸盐的主要膳食来源。通过色谱法和结晶法分离豌豆素。其结构通过质谱分析以及单糖和苷元的鉴定得到证实。 Prunasin 通过 HPLC 进行色谱鉴定。苦菜苷 (0.68 μmol/g) 和普那辛 (0.16 μmol/g) 的种子总含量相当于种子的氰含量 (0.91 ± 0.14 μmol/g;n= 7)，在自溶后测定为氰化物。当喂食维西亚宁时，尿硫氰酸盐排出量为维西亚宁摄入量的21%，而β-氰基丙氨酸产生的尿硫氰酸盐排出量<0.2%。计算表明，73%的硫氰酸盐可以源自vicianin和prunasin，其中27%源自β-氰基丙氨酸。硫氰酸盐尿排出量高与内分泌和神经系统疾病有关。硫氰酸盐；硫酸盐；普通野豌豆；野豌豆 (Vicia sativa L.)；维琴宁;普鲁那辛;豆类氰； β-氰基丙氨酸
When young rats were fed a diet containing common vetch seed for 1 month, they excreted in the urine ∼7 times more thiocyanate than they had ingested. Vicianin, prunasin, and β-cyanoalanine were identified as principal dietary sources of the excreted thiocyanate. Vicianin was isolated by chromatography and crystallization. Its structure was confirmed by mass spectrometry and by identification of its monosaccharides and aglycon. Prunasin was identified chromatographically by HPLC. The combined seed content of vicianin (0.68 μmol/g) and prunasin (0.16 μmol/g) corresponded to the cyanogen content of the seed (0.91 ± 0.14 μmol/g;n= 7), determined as cyanide after autolysis. When vicianin was fed, the urinary thiocyanate output was 21% of the ingested amount of vicianin, whereas β-cyanoalanine yielded a urinary thiocyanate output of <0.2%. Calculations show that 73% of the thiocyanate can be derived from vicianin and prunasin, with 27% derived from β-cyanoalanine. High urinary output of thiocyanate has been associated with endocrine and neurological disorders.Keywords: Cyanide; thiocyanate; sulfate; common vetch; Vicia sativa L.; vicianin; prunasin; legume cyanogen; β-cyanoalanine