The Effects of Electrically Polarized Hydroxyapatite on Osteogenic Cell Activity and Bone Formation
The Effects of Electrically Polarized Hydroxyapatite on Osteogenic Cell Activity and Bone Formation
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DOI：
10.4028/www.scientific.net/kem.309-311.153
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发表时间：
2006-01
期刊：
影响因子：
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通讯作者：
S. Itoh;Satoshi Nakamura;Takayuki Kobayashi;K. Shinomiya;K. Yamashita
中科院分区：
文献类型：
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作者：
S. Itoh;Satoshi Nakamura;Takayuki Kobayashi;K. Shinomiya;K. Yamashita
通过质子传输极化，在羟基磷灰石（HAp）陶瓷上可诱导出独特的大表面电荷。在本研究中，我们研究了成骨细胞的反应和新骨形成的电极HAp板植入到大鼠颅骨骨缺损。在第一组大鼠中，将测试件以其带正电荷的表面面朝下放置在硬脑膜上。在第二组中，将测试件以其带负电荷的表面面朝下放置在硬脑膜上。第三组接受不带电的测试件。测量骨矿物质密度，并进行组织学检查以表征新形成的骨以及定量。此外，酶组织化学检测碱性磷酸酶和抗酒石酸酸性磷酸酶进行定量成骨细胞活性。在颅骨膜处未观察到骨形成，表明颅骨膜是不良的骨诱导剂。在接受极化HAp板的组中，在带负电荷和正电荷的表面上，骨生长得到增强。除了静电力将Ca 2+吸引到带负电荷的表面之外，带正电荷的表面上对破骨细胞增殖的抑制作用可能促进体内骨形成。
Exclusively large surface charges are inducible on hydroxyapatite (HAp) ceramics by proton transport polarization. In the present study, we examined osteogenic cell responses and new bone formation on electrically polarized HAp plates that were implanted into calvarial bone defects in rats. In the first group of rats, test pieces were placed with their positively charged surfaces face down on the dura mater. In a second group, test pieces were placed with their negatively charged surfaces face down on the dura mater. A third group received non-charged test pieces. Bone mineral density was measured and histological examination was carried out to characterize the newly formed bone as well as quantification. Furthermore, enzyme histochemistry involving the detection of alkaline phosphatase and tartrate-resistant acid phosphatase was performed to quantify osteogenic cell activity. No bone formation was observed at the pericranium, suggesting that the pericranium is a poor osteoinducer. Bone growth was enhanced in the groups that received polarized HAp plates, both at the negatively and positively charged surfaces. In addition to the electrostatic force that attracts Ca 2+ to the negatively charged surfaces, the suppressive effects on osteoclasts proliferation on the positively charged surfaces may enhance bone formation in vivo.