Mucilaginibacter pedocola sp nov., isolated from a heavy-metal-contaminated paddy field
Mucilaginibacter pedocola sp nov., isolated from a heavy-metal-contaminated paddy field
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Mucilaginibacter pedocola sp nov.，从重金属污染的稻田中分离出来
DOI：
10.1099/ijsem.0.001306
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发表时间：
2016
影响因子：
2.8
通讯作者：
Wang Gejiao
中科院分区：
文献类型：
--
作者：
Tang Jingwei;Huang Jing;Qiao Zixu;Wang Rui;Wang Gejiao
菌株tbz30t从重金属污染的稻田土壤中分离得到。菌株tbz30t细胞革兰氏染色阴性，呈棒状，不运动，不形成孢子。该分离物为严格需氧菌，呈粉红色，过氧化氢酶和氧化酶阳性，并产生胞外多糖。根据16S rRNA基因系统发育，菌株tbz30t属于mucilaginibacter属，与gynuensis Mucilaginibacter yc7003t（95.8%）、litoreusmucilaginibacter br - 18t（95.4%）和malensismucilaginibacter mp1x4t（95.4%）亲缘关系最密切。菌株tbz30t仅含甲基萘醌-7。主要细胞脂肪酸包括总特征3 （C16: 1ω7cand/或iso-C15: 02-OH）、iso-C15: 0、C16: 0、iso-C17: 03-OH和C16: 1ω5c。极性脂质为磷脂酰乙醇胺、一种未识别的磷脂、两种未识别的氨基磷脂、四种未识别的氨基磷脂、三种未识别的脂质和两种未识别的糖脂。基因组DNA G+C含量为49.0 mol%。在多相分类分析的基础上，菌株tbz30代表了mucilaginibacter属的一个新种，其名称为emucilaginibacter pedocolasp。11月被提议。型应变为TBZ30T（=KCTC 42833T=CCTCC AB 2015301T）。
Strain TBZ30Twas isolated from soil of a heavy-metal-contaminated paddy field. Cells of strain TBZ30Twere Gram-staining-negative, rod-shaped, non-motile and non-spore-forming. The isolate was strictly aerobic, pink-pigmented, catalase- and oxidase-positive and produced exopolysaccharides. On the basis of 16S rRNA gene phylogeny, strain TBZ30Tbelonged to the genusMucilaginibacterand appeared most closely related toMucilaginibacter gynuensisYC7003T(95.8 %),Mucilaginibacter litoreusBR-18T(95.4 %) andMucilaginibacter mallensisMP1X4T(95.4 %). Strain TBZ30Tcontained menaquinone-7 as the only ubiquinone. The main cellular fatty acids included summed feature 3 (C16 : 1ω7cand/or iso-C15 : 02-OH), iso-C15 : 0, C16 : 0, iso-C17 : 03-OH and C16 : 1ω5c. The polar lipids were phosphatidylethanolamine, an unidentified phospholipid, two unidentified aminophospholipids, four unidentified aminolipids, three unidentified lipids and two unidentified glycolipids. The genomic DNA G+C content was 49.0 mol%. On the basis of polyphasic taxonomy analyses, strain TBZ30Trepresents a novel species of the genusMucilaginibacter, for which the nameMucilaginibacter pedocolasp. nov. is proposed. The type strain is TBZ30T(=KCTC 42833T=CCTCC AB 2015301T).