DNA microarray analyses reveal a post-irradiation differential time-dependent gene expression profile in yeast cells exposed to X-rays and γ-rays
DNA microarray analyses reveal a post-irradiation differential time-dependent gene expression profile in yeast cells exposed to X-rays and γ-rays
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DOI：
10.1016/j.bbrc.2006.05.126
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发表时间：
2006-07-21
影响因子：
3.1
通讯作者：
Iwahashi, Hitoshi
中科院分区：
文献类型：
--
作者：
Kimura, Shinzo;Ishidou, Emi;Iwahashi, Hitoshi
电离辐射（IR）是我们环境中最神秘的遗传毒性应激诱导剂，它从很久以前就存在了。IR通常被认为是有害的，并且已经成为许多研究的主题，主要关注细胞中的DNA损伤作用及其修复机制。此外，很少有研究集中在大规模的识别细胞响应IR，为此，我们在这里描述的单细胞基因组模型，酵母（酿酒酵母菌株S288 C），通过cDNA微阵列的转录反应的初步研究。通过用50戈伊剂量的X-和γ-射线照射在YPD培养基中培养的酵母细胞，然后将细胞重悬于YPD中进行时间过程实验，研究了两种不同IR、X-射线和γ（gamma）-射线的作用。在照射后20、40和80分钟收集样品用于微阵列分析。微阵列分析揭示了改变基因表达的时程转录谱。上调基因属于主要与细胞周期和DNA加工、细胞救援防御和毒力、蛋白质和细胞命运以及代谢（X射线和γ射线）相关的功能类别。同样，对于X射线和γ射线，下调的基因主要分别属于转录和蛋白质合成、细胞周期和DNA加工、细胞组织控制、细胞命运以及C化合物和碳水化合物代谢类别。本研究首次提供了X射线和γ射线照射后酵母细胞全基因组mRNA表达谱的快照，并比较解释/讨论了这两种辐射相对于其能量水平的影响所改变的基因功能类别。(c)2006年爱思唯尔公司All rights reserved.
Ionizing radiation (IR) is the most enigmatic of genotoxic stress inducers in our environment that has been around from the eons of time. IR is generally considered harmful, and has been the subject of numerous studies, mostly looking at the DNA damaging effects in cells and the repair mechanisms therein. Moreover, few studies have focused on large-scale identification of cellular responses to IR, and to this end, we describe here an initial study on the transcriptional responses of the unicellular genome model, yeast (Saccharomyces cerevisiae strain S288C), by cDNA microarray. The effect of two different IR, X-rays, and gamma (gamma)-rays, was investigated by irradiating the yeast cells cultured in YPD medium with 50 Gy doses of X- and gamma-rays, followed by resuspension of the cells in YPD for timecourse experiments. The samples were collected for microarray analysis at 20, 40, and 80 min after irradiation. Microarray analysis revealed a time-course transcriptional profile of changed gene expressions. Up-regulated genes belonged to the functional categories mainly related to cell cycle and DNA processing, cell rescue defense and virulence, protein and cell fate, and metabolism (X- and gamma-rays). Similarly, for X- and gamma-rays, the down-regulated genes belonged to mostly transcription and protein synthesis, cell cycle and DNA processing, control of cellular organization, cell fate, and C-compound and carbohydrate metabolism categories, respectively. This study provides for the first time a snapshot of the genome-wide mRNA expression profiles in X- and gamma-ray post-irradiated yeast cells and comparatively interprets/discusses the changed gene functional categories as effects of these two radiations vis-a-vis their energy levels. (c) 2006 Elsevier Inc. All rights reserved.