Monolithic stationary phases for liquid chromatography and capillary electrochromatography.
Monolithic stationary phases for liquid chromatography and capillary electrochromatography.
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DOI：
10.1016/s0021-9673(02)00072-9
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发表时间：
2002-04
期刊：
影响因子：
--
通讯作者：
Hanfa Zou;Xiaodong Huang;M. Ye;Q. Luo
中科院分区：
文献类型：
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作者：
Hanfa Zou;Xiaodong Huang;M. Ye;Q. Luo
整体固定相是通过原位聚合或柱管内固结制备的连续单一多孔结构，如果需要，表面被功能化以将其转化为具有所需色谱结合特性的吸附剂[J]。Chromatogr。[j]。近年来，整体固定相以其制备工艺简单、性质独特、性能优异，尤其在生物聚合物的分离方面受到了液相色谱和毛细管电色谱的广泛关注。本文综述了整体式固定相的制备、表征及其应用。此外，还简要讨论了单片固定相的缺点和局限性。
A monolithic stationary phase is the continuous unitary porous structure prepared by in situ polymerization or consolidation inside the column tubing and, if necessary, the surface is functionalized to convert it into a sorbent with the desired chromatographic binding properties [J. Chromatogr. A 855 (1999) 273]. Monolithic stationary phases have attracted considerable attention in liquid chromatography and capillary electrochromatography in recent years due to their simple preparation procedure, unique properties and excellent performance, especially for separation of biopolymers. This review summarizes the preparation, characterization and applications of the monolithic stationary phases. In addition, the disadvantages and limitations of the monolithic stationary phases are also briefly discussed.