Identification of cytoskeletal markers for the different microvilli and cell types of the rat vomeronasal sensory epithelium
Identification of cytoskeletal markers for the different microvilli and cell types of the rat vomeronasal sensory epithelium
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DOI：
10.1023/a:1026548020851
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发表时间：
2000-03-01
期刊：
影响因子：
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通讯作者：
Drenckhahn, D
中科院分区：
文献类型：
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作者：
Höfer, D;Shin, DW;Drenckhahn, D
哺乳动物鼻子的犁鼻器(VNO)专门用来检测信息素。推测信息素的化学感觉信号转导部位是VNO感觉上皮的犁鼻刷缘，它包含两组不同的微绒毛：(I)支持细胞的高微绒毛和(Ii)化学感受性VNO神经元的短微绒毛，这些微绒毛分支并与较长的支持细胞微绒毛的基底部混合。在光学显微镜水平上研究可能的VNO信号转导分子的亚细胞分布时，一个关键问题是清楚地区分来自VNO神经元树突状微绒毛和周围支持细胞结构的免疫信号。因此，在目前的研究中，我们寻找细胞骨架标记蛋白，这可能有助于在光学显微镜水平上区分这两组微绒毛。通过免疫组织化学染色，我们发现VNO树突状微绒毛可以选择性地被钙敏感的肌动蛋白细丝捆绑蛋白Villin的抗体标记，而支持细胞微绒毛含有肌动蛋白细丝交联蛋白Fimbrin，而不是Villin。用于细胞识别的细胞质标记分子是用于支持细胞的细胞角蛋白18和用于VNO神经元树突的β-微管蛋白。进一步的发现是，大鼠VNO的非感觉上皮含有刷状细胞，这是一种似乎参与肠道化学接收的某些方面的细胞类型。犁鼻刷缘的刷状细胞或其他结构不含α-GUSTDEIN。
The vomeronasal organ (VNO) of the mammal nose is specialized to detect pheromones. The presumed site of the chemosensory signal transduction of pheromones is the vomeronasal brush border of the VNO sensory epithelium, which has been shown to contain two different sets of microvilli: (i) the tall microvilli of supporting cells and (ii) the short microvilli of the chemoreceptive VNO neurons that branch and intermingle with the basal portions of the longer supporting cell microvilli. A key problem when studying the subcellular distribution of possible VNO signal transduction molecules at the light microscope level is the clear discrimination of immunosignals derived from dendritic microvilli of the VNO neurons and surrounding supporting cell structures. In the present study we therefore looked for cytoskeletal marker proteins, that might help to distinguish at the light microscope level between the two sets of microvilli. By immunostaining we found that the VNO dendritic microvilli can be selectively labelled with antibodies to the calcium-sensitive actin filament-bundling protein villin, whereas supporting cell microvilli contain the actin filament cross-linking protein fimbrin, but not villin. Useful cytoplasmic marker molecules for cellular discrimination were cytokeratin 18 for supporting cells and beta -tubulin for dendrites of VNO neurons. A further finding was that the non-sensory epithelium of the rat VNO contains brush cells, a cell type that appears to be involved in certain aspects of chemoreception in the gut. Brush cells or other structures of the vomeronasal brush border did not contain alpha -gustducin.