CARBON-DIOXIDE AS A SHORT-TERM RESTRAINT ANESTHETIC IN RATS WITH SUBCLINICAL RESPIRATORY-DISEASE
CARBON-DIOXIDE AS A SHORT-TERM RESTRAINT ANESTHETIC IN RATS WITH SUBCLINICAL RESPIRATORY-DISEASE
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DOI：
10.1258/002367789780810590
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发表时间：
1989-07-01
影响因子：
2.4
通讯作者：
BLACKSHAW, JK
中科院分区：
文献类型：
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作者：
FENWICK, DC;BLACKSHAW, JK
在3个独立的实验中评估了二氧化碳（CO2）与和不与氧气（O2）一起作为Wistar大鼠的短期束缚麻醉剂的使用，其中亚临床呼吸系统疾病是地方病。首先，将大鼠置于70 l塑料箱中由固体CO2芯片产生的CO2气氛中，并在踏板反射消失后以0至120 s的时间间隔取出。25只大鼠中有8只死亡，其中2只在踏板反射消失后立即被移除;得出结论，不含O2的CO2对大鼠来说不是合适的短期麻醉剂。在第二项研究中，大鼠在市售气瓶提供的50：50和80：20（CO2：O2）气氛中麻醉，在2个不同的环境中-3.41玻璃罐和171塑料箱。老鼠在塑料箱中变得兴奋，而不是玻璃罐。玻璃罐中的大鼠在80：20（CO2：O2）中比在50：50混合物中（4.2 ± 0.5）明显更快地表现出明显的抑郁和胡须运动的停止。0.98 s，n = 6和66.0 ± 4.9 s，n - 6 vs 13.8 ±。2.77 s，n=6，66.0 ±。4.9 s，n = 6 vs 13.8 ±。2.77 s，n = 5和152.0。20.8 s，n= 5）。在171塑料箱中的大鼠在平均30.9 ±. 6.38 80：20（CO_2：O_2）组11例。那些留在50：50混合物中60秒和180秒后，他们的踏板反射消失，恢复这些反射在20.2 .+-。0.44 s和21.5 .+-. 7.23 s分别从气体中去除后。在13 min 10 s后，在50：50混合物中的一只大鼠中引起呼吸和心跳。大鼠在50：50混合物中放置180 s后，足反射消失，未发生不良反应，但在80：20混合物中放置相同时间后，2只死亡。在第三项研究中，描述了50：50混合物作为短期束缚麻醉剂的实际使用示例。得出的结论是，这种混合物是一种廉价，安全和有效的手段，短期约束大鼠与亚临床呼吸系统疾病，当暴露于气体的最小时间。
the use of carbon dioxide (CO2) with, and without oxygen (O2) as a short-term restraint anaesthetic for Wistar rats in which subclinical respiratory disease was endemic, was assessed in 3 separate experiments. In the first, rats were placed in a CO2 atmosphere generated from solid CO2 chips in a 70l plastic bin, and removed at time intervals ranging from 0 to 120s after disappearance of the pedal reflex. Eight of 25 rats died, including 2 which were removed immediately the pedal reflex disappeared; it was concluded that CO2 without O2 was not a suitable short-term anaesthetic for rats. In a second study, rats were anaesthetized in atmospheres of 50:50 and 80:20 (CO2: O2) provided from commercially available cylinders, in 2 different environments- a 3.41 glass jar and a 171 plastic bin. Rats became excited in the plastic bin but not the glass jar. Rats in the glass jar displayed visible depression and cessation of whiskers movement significantly more quickly in the 80:20 (CO2: O2) than in the 50: 50 mixture (4.2 .+-. 0.98 s, n = 6, and 66.0 4.9 s, n - 6 vs 13.8 .+-. 2.77 s, n=6, and 66.0 .+-. 4.9 s, n = 6 vs 13.8 .+-. 2.77 s, n = 5 and 152.0 .+-. 20.8 s, n= 5, respectively). Rats in the 171 plastic bin lost their pedal reflexes in a mean 30.9 .+-. 6.38 s (n = 11) in the 80:20 (CO2:O2) group. Those left in the 50:50 mixture for 60s and 180s after disappearance of their pedal reflexes, recovered these reflexes in 20.2 .+-. 0.44 s and 21.5 .+-. 7.23 s respectively after removal from the gas. Respiration and heart beat caused in one rat remaining in the 50:50 mixture after 13 imin 10 s. No untoward effects occurred in rats left in the 50:50 mixture for 180 s after disappearance of the pedal reflex, but 2 died when left for an equivalent period in the 80:20 mixture. In the third study, examples of the practical use of a 50:50 mixture as a short term restraint anaesthetic are described. It was concluded that this mixture was a cheap, safe, and effective means of short-term restraint for rats with subclinical respiratory disease, when the minimal time of exposure to the gases was employed.