Does local injection of reveromycin A inhibit tooth movement without causing systemic side effects?
Does local injection of reveromycin A inhibit tooth movement without causing systemic side effects?
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DOI：
10.1093/ejo/cjaa067
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发表时间：
2021-03-19
影响因子：
2.6
通讯作者：
Goto, Shigemi
中科院分区：
文献类型：
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作者：
Kako, Shunsuke;Tabuchi, Masako;Goto, Shigemi
目的：在实验性牙齿移动的模型小鼠中，通过局部口内注射逆转霉素A（RMA）来确定局部抑制破骨细胞活性和控制牙齿移动的可行性。8周龄野生型小鼠（每组n = 6）分为四组，包括两个非RMA组，接受生理盐水14（14天非RMA组）或连续21天（21天非RMA组）和2个RMA组（14天RMA组）或连续21天（21天RMA组）接受RMA（1.0 mg/kg体重）。RMA局部注射到左侧上颌第一磨牙的颊粘膜中，每天两次，从放置10 gf镍钛闭合螺旋弹簧前3天开始。使用微型计算机断层扫描分析牙齿移动距离。对周围牙槽骨的影响进行了评估，通过测量骨表面积与组织表面积的比例与苏木精染色切片和计数破骨细胞在牙周组织与TRAP染色切片。进行血液检查，测量胫骨颈的骨体积和骨小梁分离，以分析全身副作用。局部注射RMA抑制牙齿移动40.6%，促进牙槽骨体积维持37.4%，并在21天时抑制牙根周围破骨细胞活性40.8%。结论：在小鼠模型中局部注射RMA能够控制牙齿移动而没有全身副作用。
Objective: To determine the feasibility of local inhibition of osteoclast activity and control of tooth movement with local intraoral reveromycin A (RMA) injection in model mice for experimental tooth movement.Materials and methods: Eight-week-old wild-type mice (n = 6 per group) were divided into four groups consisting of two non-RMA groups that received normal saline for 14 (14-day non-RMA group) or 21 consecutive days (21-day non-RMA group) and 2 RMA groups that received RMA (1.0 mg/kg of weight) for 14 (14-day RMA group) or 21 consecutive days (21-day RMA group). RMA was injected locally into the buccal mucosa of the left first maxillary molar twice daily starting 3 days before placement of the 10-gf Ni-Ti closed coil spring. Tooth movement distance was analysed using micro-computed tomography. The effects on surrounding alveolar bone were evaluated by measuring the ratio of bone surface area to tissue surface area with haematoxylineosin-stained sections and counting the number of osteoclasts in periodontal tissue with TRAP-stained sections. Blood tests were performed and bone volume and trabecular separation at the tibial neck were measured to analyse systemic side effects.Results: Local RMA injection inhibited tooth movement by 40.6 per cent, promoted alveolar bone volume maintenance by 37.4 per cent, and inhibited osteoclast activity around the tooth root at 21 days by 40.8 per cent. Systemic effects on osteoclasts or osteoblasts were not observed.Conclusion: Local injection of RMA enabled control of tooth movement without systemic side effects in a mouse model.