The MHC reactivity of the T cell repertoire prior to positive and negative selection
The MHC reactivity of the T cell repertoire prior to positive and negative selection
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DOI：
10.1016/s0092-8674(00)81905-4
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发表时间：
1997-03-07
期刊：
影响因子：
64.5
通讯作者：
Raulet, DH
中科院分区：
文献类型：
--
作者：
Zerrahn, J;Held, W;Raulet, DH
成熟T细胞上的T细胞抗原受体（TCR）与自身MHC蛋白呈递的肽抗原反应，并且还经常与外源MHC蛋白交叉反应。MHC的反应性是否是固有的生殖系TCR序列或施加胸腺选择的根本问题，在这里解决诱导非选择性成熟的未成熟胸腺细胞在没有MHC分子。在预选剧目中的MHC反应性是非常高的，但不高于在正常剧目。在预选和MHC-选择的库中，克隆与多个MHC分子的交叉反应性以相似的程度发生。结果建立了生殖系TCR库的MHC反应性，表明必须经历负选择的未成熟胸腺细胞的最小分数，并表明一些TCR-MHC接触可能是保守的。
T cell antigen receptors (TCRs) on mature T cells react with peptide antigens presented by self-MHC proteins and also frequently cross-react with foreign MHC proteins. The fundamental question whether MHC reactivity is inherent in the germline TCR sequences or is imposed by thymic selection was addressed here by inducing nonselective maturation of immature thymocytes in the absence of MHC molecules. MHC reactivity in the preselection repertoire is very high, but no higher than in the normal repertoire. cross-reactivity of clones with multiple MHC molecules occurred to a similar extent in the preselection and MHC-selected repertoires. The results establish the MHC reactivity of the germline TCR repertoire, indicate the minimum fraction of immature thymocytes that must undergo negative selection, and suggest that some TCR-MHC contacts may be conserved.