Knob-positive and knob-negative Plasmodium falciparum differ in expression of a strain-specific malarial antigen on the surface of infected erythrocytes.
Knob-positive and knob-negative Plasmodium falciparum differ in expression of a strain-specific malarial antigen on the surface of infected erythrocytes.
复制标题
旋钮阳性和旋钮阴性疟原虫恶性疟原虫在感染红细胞表面的菌株特异性疟疾抗原的表达上有所不同。
DOI：
10.1084/jem.160.5.1585
复制
发表时间：
1984-11-01
影响因子：
15.3
通讯作者：
Howard, R J
中科院分区：
文献类型：
--
作者：
Aley, S B;Sherwood, J A;Howard, R J
我们研究了恶性疟原虫结节阳性(K+)株的无结变异体(K-)感染红细胞表面的一种品系特异性疟疾抗原的表达。用乳过氧化物酶方法对感染了两个独立地理分离物(马来亚营地和圣露西亚)的K+或K-寄生虫的Aotus血液进行表面碘化。然后用一种新的方法分离感染和未感染的红细胞，该方法包括在含有山梨醇的Percoll梯度上进行平衡密度沉淀。根据K+菌株的特征大小和洗涤剂的溶解性，可以很容易地在感染K+菌株的红细胞表面识别出菌株特异性抗原。在感染K变异体的红细胞表面以及从K+或K感染的血液中分离的未感染的红细胞上均未检测到这些蛋白。这些结果与品系特异性表面抗原在恶性疟原虫感染的红细胞细胞黏附中的作用一致。我们的发现代表了K+和K-P.恶性疟原虫之间的第二个生化差异(与结节相关的富含组氨酸的蛋白)。
We have investigated the expression of a strain-specific malarial antigen on the surface of erythrocytes infected with knobless (K-) variants of knob-positive (K+) strains of Plasmodium falciparum. Aotus blood infected with K+ or K- parasites derived from two independent geographical isolates (Malayan camp and Santa Lucia) was surface iodinated by the lactoperoxidase method. Infected and uninfected erythrocytes were then separated by a new procedure involving equilibrium density sedimentation on a Percoll gradient containing sorbitol. Strain-specific antigens were readily identified on the surface of erythrocytes infected with either of the K+ strains by their characteristic size and detergent solubility. These proteins were not detected on the surface of erythrocytes infected with either of the K- variants nor on uninfected erythrocytes isolated from K+- or K- - infected blood. These results are consistent with a role for the strain- specific surface antigen in cytoadherence of P. falciparum-infected erythrocytes. Our findings represent the second biochemical difference (with the knob-associated histidine-rich protein) between K+ and K- P. falciparum.