PRODUCTION OF BACTERIOPHAGE MUTANTS BY A DISTURBANCE OF DEOXYRIBONUCLEIC ACID METABOLISM
PRODUCTION OF BACTERIOPHAGE MUTANTS BY A DISTURBANCE OF DEOXYRIBONUCLEIC ACID METABOLISM
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DOI：
10.1038/178529b0
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发表时间：
1956-01-01
期刊：
影响因子：
64.8
通讯作者：
PARDEE, AB
中科院分区：
文献类型：
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作者：
LITMAN, RM;PARDEE, AB
N个实验中，嘧啶衍生物|5.根据Dunn和Smith的方法，将溴尿嘧啶掺入T2 r噬菌体的脱氧核糖核酸中，观察到在子代噬菌体中出现了大量的各种噬斑型突变体，如图1所示。观察到5-溴尿嘧啶的这种明显的强诱变作用，使我们对产生噬菌体突变体所依赖的条件进行了研究。在一个典型的实验中，大肠杆菌菌株B的培养物在37 ℃下通气生长.在补充有50 gm./ ml. 5.布仑尿嘧啶，传代4代至2 μ 108个细胞/ml的密度。（完全培养基是葡萄糖盐培养基吗？补充l mgm./ ml.磺胺，1毫克/ ml.不含维生素的酪蛋白水解物，25 pgm./ ml.黄嘌呤和尿嘧啶等于所添加的任何5-溴尿嘧啶的5.5%。我们发现，当商业5-溴尿嘧啶，
N experiments in which the pyrimidine derivative| 5. bromouracil was incorporated into the deoxyribonucleic acid of T2r" bacteriophage, according to the method of Dunn and Smith", it was observed that, a large number of a variety of plaque-type mutants appeared among the progeny phages, as illustrated in Fig. 1. The observation of this appar-ently powerful mutagenic effect of 5-bromouracil led us to an investigation of the conditions upon which the production of phage mutant depends. In a typical experiment, a culture of Escherichia, coli, strain B, was grown with aeration at 37 C. in complete medium supplemented with 50 gm./ml. 5. bronnouracil, for four generations to a density of 2 y 108 cells/ml.(Complete medium is a glucosesalts medium? supplemented with l mgm./ml. sulphanilamide, 1 mgm./ml. vitamin-free casein hydrolysate, 25 pgm./ml. xanthine, and uracil equal 5 5 per cent of any 5-bromouracil added. We had found that when commercial 5-bromouracil, which